Iris Severe Mosaic Virus (ISMV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V176
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all ISMV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.600 - 2.300 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues such as iris and other host plants. Background is low on all of the negative control wells.
Application: The test can be used to detect ISMV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells | Price $US |
Ship
Weight (Estimated) |
|
V176-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
250 |
2 lb (1 kg) |
|
V176-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
425 |
3 lb (1.5 kg) |
|
V176-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1690 |
12 lb (6 kg) |
| V176-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
95 |
1 lb (0.5 kg) |
| V176-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
385 |
2 lb (1 kg) |
| V176-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
610 |
3 lb (1.5 kg) |
|
V176-C1 |
Coating Antibody |
500 |
125 |
2 lb (1 kg) |
|
V176-C2 |
Coating Antibody |
1000 |
235 |
2 lb (1 kg) |
|
V176-C3 |
Coating Antibody |
5000 |
805 |
2 lb (1 kg) |
|
V176-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
170 |
2 lb (1 kg) |
|
V176-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
285 |
2 lb (1 kg) |
|
V176-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
980 |
2 lb (1 kg) |
|
V176-P1 |
Positive control |
18 |
15 |
1 lb (0.5 kg) |
|
V176-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 |
2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 |
3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 |
6 lb (3.0 kg) |
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An Order
To know more about the products: Product
Items
To learn how to perform the test:
Instructions for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Iris Severe Mosaic Virus
Acronym: ISMV
Synonyms: bearded iris mosaic virus (Brunt et al., 1988),
iris grijs virus, iris stripe virus, iris yellow mosaic virus
Group/Genus: Potyviruses
Vector: Transmitted by a vector; an insect; Macrosiphum euphorbiae
and Myzus persicae, but not by Myzus pelargonii, M. circumflexum
or Anuraphis tulipae and Rhopalosiphum tulipaella (Brierley and
McWhorter, 1934; Brierley and Smith, 1948; Travis, 1957; Barnett et al.,
1971; Asjes, 1979); Aphididae. Transmitted in a non-persistent manner. .
Transmission: Virus transmitted by mechanical inoculation; not
transmitted by grafting; not transmitted by contact between plants.
Main host plants: Crocus vernus, Belamcanda chinensis, Iris
spp.
Virus Infection: Cause symptoms of leaf striping, stunting, flower
breaking. Plants die prematurely, leaf mosaic; striping of flowers, if not white
or yellowl,.or severe chlorosis. Symptoms persist and vary cyclically
over a few weeks.
Diagnostically susceptible host species and symptoms: Belamcanda
chinensis, Iris × hollandica. No herbaceous hosts.
Geographical distribution: Spreads in Australia, the former Czechoslovakia, Denmark, Japan, the Netherlands, Sweden, the UK, and the USA (and probably wherever bulbous and rhizomatous irises and crocus are grown).
References:
1. Barnett, O.W. and Brunt, A.A. (1975). CMI/AAB Descr. Pl. Viruses
No. 147, 4 pp.
2. Hammond, J., Lawson, R.H. and Hsu, T. (1985). Phytopathology
75: 1353.