Iris Severe Mosaic Virus (ISMV) - DAS ELISA                             Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V176
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all ISMV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.600 - 2.300 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as iris and other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect ISMV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V176-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V176-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V176-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V176-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V176-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V176-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V176-C1

Coating Antibody

500

125

2 lb (1 kg)

V176-C2

Coating Antibody

1000

235

2 lb (1 kg)

V176-C3

Coating Antibody

5000

805

2 lb (1 kg)

V176-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V176-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V176-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V176-P1

Positive control

18

18

1 lb (0.5 kg)

V176-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
   Instructions for DAS ELISA
To be familiar with other components:  Buffer Sets; Controls

Information About the Virus

Name:  Iris Severe Mosaic Virus
Acronym:  ISMV
Synonyms:  bearded iris mosaic virus (Brunt et al., 1988), iris grijs virus, iris stripe virus, iris yellow mosaic virus
Group/Genus:  Potyviruses

Vector:  Transmitted by a vector; an insect; Macrosiphum euphorbiae and Myzus persicae, but not by Myzus pelargonii, M. circumflexum or Anuraphis tulipae and Rhopalosiphum tulipaella (Brierley and McWhorter, 1934; Brierley and Smith, 1948; Travis, 1957; Barnett et al., 1971; Asjes, 1979); Aphididae. Transmitted in a non-persistent manner. .
Transmission: Virus transmitted by mechanical inoculation; not transmitted by grafting; not transmitted by contact between plants.
Main host plants:  Crocus vernus, Belamcanda chinensis, Iris spp.

Virus Infection:  Cause symptoms of leaf striping, stunting, flower breaking. Plants die prematurely, leaf mosaic; striping of flowers, if not white or yellowl,.or severe chlorosis. Symptoms persist and vary cyclically over a few weeks.
Diagnostically susceptible host species and symptoms:
Belamcanda chinensis, Iris hollandica. No herbaceous hosts.

Geographical distribution: Spreads in Australia, the former Czechoslovakia, Denmark, Japan, the Netherlands, Sweden, the UK, and the USA (and probably wherever bulbous and rhizomatous irises and crocus are grown).

 References:
1. 
Barnett, O.W. and Brunt, A.A. (1975). CMI/AAB Descr. Pl. Viruses No. 147, 4 pp.
2.  Hammond, J., Lawson, R.H. and Hsu, T. (1985). Phytopathology 75: 1353.


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