Hibiscus Chlorotic Ringspot Virus (HCRSV) - DAS ELISA                Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V153
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all HCRSV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.800 - >2.600 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as hibiscus, or other host plants. Background is relative low on all of  the negative control wells.

Application:  The test can be used to detect HCRSV in infected host plant tissues.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

V153-R1

ELISA Reagents, Alkaline Phosphatase

500

230

2 lb (1 kg)

V153-R2

ELISA Reagents, Alkaline Phosphatase

1000

395

3 lb (1.5 kg)

V153-R3

ELISA Reagents, Alkaline Phosphatase

5000

1590

12 lb (6 kg)
V153-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V153-K1 ELISA Kit, Alkaline Phosphatase

500

345

2 lb (1 kg)
V153-K2 ELISA Kit, Alkaline Phosphatase

1000

535

3 lb (1.5 kg)

V153-C1

Coating Antibody

500

115

2 lb (1 kg)

V153-C2

Coating Antibody

1000

205

2 lb (1 kg)

V153-C3

Coating Antibody

5000

725

2 lb (1 kg)

V153-D1

Detecting conjugate, Alkaline Phosphatase

500

140

2 lb (1 kg)

V153-D2

Detecting conjugate, Alkaline Phosphatase

1000

225

2 lb (1 kg)

V153-D3

Detecting conjugate, Alkaline Phosphatase

5000

900

2 lb (1 kg)

V153-P2

ELISA control, positive

18

15

1 lb (0.5 kg)

V153-N1

Negative control

18

15

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500 100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000  130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000  400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Hibiscus Chlorotic Ringspot Virus
Acronym:  HCRSV
Synonyms:  
Group/Genus:  Carmovirus

Vector:  Transmitted by means not involving a vector.
Transmission: Transmitted by mechanical inoculation.
Main natural host plants: Hibiscus rosa-sinensis; Abelmoschus manihot.

Virus Infection:  Symptoms by viral infection include mottling or chlorotic spots, rings, vein-banding or mosaic, very variable. Symptoms persist.
Diagnostically susceptible host species and symptoms: Chenopodium quinoa - chlorotic local lesions, not systemic; Cyamopsis tetragonoloba, Gossypium hirsutum, Vigna unguiculata ssp. cylindrica - necrotic local lesions, not systemic; Hibiscus cannabinus (kenaf) - ringspots, local lesions, then systemic mottle; Phaseolus vulgaris cv. Pinto - small local lesions, not systemic.

Geographical distribution: Spreads in Australia, El Salvador, Fiji, the Solomon Islands, Thailand, and the USA.

References:
1. 
Brunt, A.A., Barton, R.J., Phillips, S. and Lana, A.O. (1980). Ann. appl. Biol. 96: 37.
2.  Waterworth, H.E. (1980). CMI/AAB Descr. Pl. Viruses No. 227, 4 pp.


Home    Products    Testing Services   Supplies   Contact Us

nostics, plant pathogen detection, plant disease detection, plant virus detection, agri-diagnostics, tests for plant viruses, virus, plant bacterium, bacteria, ELISA tests for plant pathogens, Agdia, Plant diagnostics, plant diagnosis, Plant pathogen, plant pathogens, pathogen detection, laboratory services, ELISA Reagents, reagents, test kits, testing kit, antibody, antibodies, plant virus, plant viruses, virus detection, plant bacterium, plant bacteria, bacterial detection, fungus, fungi, fungal detection, testing services, virus disease, bacterial disease, plant diseases, symptoms, controls, plant clinic, plant seed, plant seeds, seed testing, seed pathogens, tomato diseases, ornamental diseases, potato diseases, seed pathogens, virus, viruses, AMV, ApMV, BCMV, BYDV, CMC, LMV, INSV, PLRV, PVA, PVM,PVS, PVX, PVY, POTY, PNYSV, SMV, SqMV, TAV, TMV, ToMV, TRSV, ToRSV, TSV, TSWV, TuMV, ZYMV, Plant pathogen detection, ELISA Reagents or ELISA kits for detection of plant pathogens, plant virus detection, plant bacterial detection, fungus detection, test services for plant pathogens, virus disease test, plant disease clinic, plant seed tests, seed test for pathogens, potato pathogen detection, tomato pathogen detection, ornamental pathogen detection, test for potato viruses, test for ornamental viruses, test for seed borne pathogens, plant bacteria, plant viruses, plant pathogens, plant disease, AC is a plant diagnostic company. AC Diagnostics provides ELISA Reagents and ELISA