Hibiscus Chlorotic Ringspot Virus (HCRSV) - DAS ELISA                Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V153
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all HCRSV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.800 - >2.600 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as hibiscus, or other host plants. Background is relative low on all of  the negative control wells.

Application:  The test can be used to detect HCRSV in infected host plant tissues.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

V153-R1

ELISA Reagents, Alkaline Phosphatase

500

230

2 lb (1 kg)

V153-R2

ELISA Reagents, Alkaline Phosphatase

1000

395

3 lb (1.5 kg)

V153-R3

ELISA Reagents, Alkaline Phosphatase

5000

1590

12 lb (6 kg)
V153-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V153-K1 ELISA Kit, Alkaline Phosphatase

500

345

2 lb (1 kg)
V153-K2 ELISA Kit, Alkaline Phosphatase

1000

535

3 lb (1.5 kg)

V153-C1

Coating Antibody

500

115

2 lb (1 kg)

V153-C2

Coating Antibody

1000

205

2 lb (1 kg)

V153-C3

Coating Antibody

5000

725

2 lb (1 kg)

V153-D1

Detecting conjugate, Alkaline Phosphatase

500

140

2 lb (1 kg)

V153-D2

Detecting conjugate, Alkaline Phosphatase

1000

225

2 lb (1 kg)

V153-D3

Detecting conjugate, Alkaline Phosphatase

5000

900

2 lb (1 kg)

V153-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V153-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500 100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000  130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000  400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Hibiscus Chlorotic Ringspot Virus
Acronym:  HCRSV
Synonyms:  
Group/Genus:  Carmovirus

Vector:  Transmitted by means not involving a vector.
Transmission: Transmitted by mechanical inoculation.
Main natural host plants: Hibiscus rosa-sinensis; Abelmoschus manihot.

Virus Infection:  Symptoms by viral infection include mottling or chlorotic spots, rings, vein-banding or mosaic, very variable. Symptoms persist.
Diagnostically susceptible host species and symptoms: Chenopodium quinoa - chlorotic local lesions, not systemic; Cyamopsis tetragonoloba, Gossypium hirsutum, Vigna unguiculata ssp. cylindrica - necrotic local lesions, not systemic; Hibiscus cannabinus (kenaf) - ringspots, local lesions, then systemic mottle; Phaseolus vulgaris cv. Pinto - small local lesions, not systemic.

Geographical distribution: Spreads in Australia, El Salvador, Fiji, the Solomon Islands, Thailand, and the USA.

References:
1. 
Brunt, A.A., Barton, R.J., Phillips, S. and Lana, A.O. (1980). Ann. appl. Biol. 96: 37.
2.  Waterworth, H.E. (1980). CMI/AAB Descr. Pl. Viruses No. 227, 4 pp.


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