Grapevine Leafroll-Associated Virus - 3 (GLRaV-3) - DAS ELISA      Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V209
Source Antibody:  Rabbit polyclonal antibodies for both capture and detection..
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all GLRaV-3 isolates tested. Reaction of the ELISA is strong.  Optical Density at 405nm  is in arrange of 0.580 - >2.950 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted  at 1:30 -1:270.

Specificity:  There is no cross reaction with healthy plant  tissues such as grapevine or other host plants. Background is moderate low on all of  the negative control wells.

Application:  The test can be used to detect GLRaV-3 in infected host plant tissues.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

V209-R1

ELISA Reagents, Alkaline Phosphatase

500

290

2 lb (1 kg)

V209-R2

ELISA Reagents, Alkaline Phosphatase

1000

505

3 lb (1.5 kg)

V209-R3

ELISA Reagents, Alkaline Phosphatase

5000

2015

12 lb (6 kg)
V209-R4 ELISA Reagents, Alkaline Phosphatase

100

105

1 lb (0.5 kg)
V209-K1 ELISA Kit, Alkaline Phosphatase

500

435

2 lb (1 kg)
V209-K2 ELISA Kit, Alkaline Phosphatase

1000

670

3 lb (1.5 kg)

V209-C1

Coating Antibody

500

145

2 lb (1 kg)

V209-C2

Coating Antibody

1000

260

2 lb (1 kg)

V209-C3

Coating Antibody

5000

915

2 lb (1 kg)

V209-D1

Detecting conjugate, Alkaline Phosphatase

500

180

2 lb (1 kg)

V209-D2

Detecting conjugate, Alkaline Phosphatase

1000

295

2 lb (1 kg)

V209-D3

Detecting conjugate, Alkaline Phosphatase

5000

1155

2 lb (1 kg)

V209-P2

ELISA control, positive

18

20

1 lb (0.5 kg)

V209-N1

Negative control

18

20

1 lb (0.5 kg)
M029-1 Buffers for DAS/TAS, for grape tests, alkaline phosphatase
500
100
2 lb (1.0 kg)
M029-2 Buffers for DAS/TAS, for grape tests, alkaline phosphatase
1000 
130
3 lb (1.5 kg)
M029-3 Buffers for DAS/TAS, for grape tests, alkaline phosphatase
5000 
400
6 lb (3.0 kg)

It is asy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Grapevine Leafroll Associated Virus - 3
Acronym:  GLRaV-3
Synonyms:  Six viruses, designated grapevine leafroll-associated 1,2,3,5,6 and 7 closteroviruses have been associated with grapevine leafroll disease.
Group/Genus:  Closterovirus

Vector:  Transmitted by Pseudococcus longispinus, Planococcus ficus (GLRaV-3).
Transmission: Virus is not transmitted by mechanical inoculation. Virus transmitted by grafting.
Main natural host plants: Vitis vinifera.

Virus Infection Symptoms:  Rolling and discoloration of leaves (reddish-purple in red-fruited, and yellowish in white-fruited cultivars), especially conspicuous in mate summer and early autumn in intolerant cultivars. Symptomless infection in some American Vitis spp. and hybrids.
Diagnostically susceptible host species and symptoms: Vitis vinifera cvs Cabernet, Pinot Noir, Mission, Gamay - leafrolling and discoloration.

Geographical distribution: Probably distributed worldwide.

References:
1. 
Candresse, T. and Martelli, G.P. (1995). Arch. Virol., Suppl. 10: 461.
2.  Hu, J.S., Gonsalves, D. and Teliz, D. (1990). J. Phytopathology 128: 1.
3.  Faoro, F., Tornaghi, R., Cinquanta, S. and Belli, G. (1992). Riv. Pat. Veg. SV 2: 67.


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