Grapevine Leafroll-Associated Virus - 3 (GLRaV-3) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V209
Source Antibody: Rabbit polyclonal antibodies for both capture and
detection..
Test Format: DAS ELISA
Reactivity: The test reacted with all GLRaV-3 isolates tested. Reaction of the ELISA is strong. Optical Density at 405nm is in arrange of 0.580 - >2.950 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:30 -1:270.
Specificity: There is no cross reaction with healthy plant tissues such as grapevine or other host plants. Background is moderate low on all of the negative control wells.
Application: The test can be used to detect GLRaV-3 in infected host plant tissues.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
|
V209-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
290 |
2 lb (1 kg) |
|
V209-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
505 |
3 lb (1.5 kg) |
|
V209-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
2015 |
12 lb (6 kg) |
| V209-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
105 |
1 lb (0.5 kg) |
| V209-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
425 |
2 lb (1 kg) |
| V209-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
660 |
3 lb (1.5 kg) |
|
V209-C1 |
Coating Antibody |
500 |
145 |
2 lb (1 kg) |
|
V209-C2 |
Coating Antibody |
1000 |
260 |
2 lb (1 kg) |
|
V209-C3 |
Coating Antibody |
5000 |
915 |
2 lb (1 kg) |
|
V209-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
180 |
2 lb (1 kg) |
|
V209-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
285 |
2 lb (1 kg) |
|
V209-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
1135 |
2 lb (1 kg) |
|
V209-P2 |
ELISA control, positive |
18 |
20 |
1 lb (0.5 kg) |
|
V209-N1 |
Negative control |
18 |
20 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 105 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 135 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 425 | 6 lb (3.0 kg) |
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Items
To learn how to perform the test: Instructions
for DAS ELISA Reagents
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Grapevine Leafroll Associated Virus - 3
Acronym: GLRaV-3
Synonyms: Six viruses, designated grapevine leafroll-associated 1,2,3,5,6
and 7 closteroviruses have been associated with grapevine leafroll disease.
Group/Genus: Closterovirus
Vector: Transmitted by Pseudococcus longispinus, Planococcus ficus
(GLRaV-3).
Transmission: Virus is not transmitted by mechanical inoculation. Virus
transmitted by grafting.
Main natural host plants: Vitis vinifera.
Virus Infection Symptoms: Rolling and discoloration
of leaves (reddish-purple in red-fruited, and yellowish in white-fruited
cultivars), especially conspicuous in mate summer and early autumn in intolerant
cultivars. Symptomless infection in some American Vitis spp. and hybrids.
Diagnostically susceptible host species and symptoms: Vitis vinifera
cvs Cabernet, Pinot Noir, Mission, Gamay - leafrolling and discoloration.
Geographical distribution: Probably distributed worldwide.
References:
1. Candresse, T. and Martelli, G.P. (1995). Arch. Virol., Suppl.
10: 461.
2. Hu, J.S., Gonsalves, D. and Teliz, D. (1990). J.
Phytopathology 128: 1.
3. Faoro, F., Tornaghi, R., Cinquanta, S. and Belli, G. (1992). Riv.
Pat. Veg. SV 2: 67.