Grapevine Fleck Virus (GFkV) - TAS ELISA                              Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V214
Source Antibody:  A rabbit polyclonal antibody for capture and and a monoclonal antibody as detection.
Test Format:  TAS ELISA                                   

Reactivity:  The test reacted with all GFkV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.410 - >2.300 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:10 -1:270.

Specificity:  There is no cross reaction with healthy plant  tissues such as grapevine or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect GFkV in infected grapevine host plant tissues.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

V214-R1

ELISA Reagents, Alkaline Phosphatase

500

290

2 lb (1 kg)

V214-R2

ELISA Reagents, Alkaline Phosphatase

1000

505

3 lb (1.5 kg)

V214-R3

ELISA Reagents, Alkaline Phosphatase

5000

2015

12 lb (6 kg)
V214-R4 ELISA Reagents, Alkaline Phosphatase

100

105

1 lb (0.5 kg)
V214-K1 ELISA Kit, Alkaline Phosphatase

500

435

2 lb (1 kg)
V214-K2 ELISA Kit, Alkaline Phosphatase

1000

670

3 lb (1.5 kg)

V214-C1

Coating Antibody

500

145

2 lb (1 kg)

V214-C2

Coating Antibody

1000

260

2 lb (1 kg)

V214-C3

Coating Antibody

5000

915

2 lb (1 kg)

V214-D1

Detecting conjugate, Alkaline Phosphatase

500

180

2 lb (1 kg)

V214-D2

Detecting conjugate, Alkaline Phosphatase

1000

295

2 lb (1 kg)

V214-D3

Detecting conjugate, Alkaline Phosphatase

5000

1155

2 lb (1 kg)

V214-P2

ELISA control, positive

18

20

1 lb (0.5 kg)

V214-N1

Negative control

18

20

1 lb (0.5 kg)
M029-1 Buffers for DAS/TAS, for grape tests, alkaline phosphatase
500
100
2 lb (1.0 kg)
M029-2 Buffers for DAS/TAS, for grape tests, alkaline phosphatase
1000 
130
3 lb (1.5 kg)
M029-3 Buffers for DAS/TAS, for grape tests, alkaline phosphatase
5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instruction for TAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Grapevine Fleck Virus
Acronym:  GFkV
Synonyms:  grapevine marbrure virus (Vuittenez et al., 1966), grapevine phloem-limited isometric virus (Boulila et al., 1990).
Group/Genus:

Vector: 
Transmission: Virus not transmitted by mechanical inoculation; transmitted by grafting.
Main natural host plants: Vitis vinifera.

Virus Infection Symptoms:  symptomless infection in most European cultivars and American Vitis species and their hybrids.
Diagnostically susceptible host species and symptoms: Vitis rupestris - scattered clearing of veinlets and leaf deformation with severe strains.

Geographical distribution: Spreads in South Africa. Found in European countries and the U.S.A.

References:
1. 
Boulila, M., Boscia, D., di Terlizzi, B., Castellano, M.A., Minafra, A., Savino, V. and Martelli, G.P. (1990). J. Phytopath. 129: 151.
2.  Engelbrecht, D.J. and Kasdorf, G.G.F. (1990). Phytophylactica 22: 347.
3.  Hewitt, W.B., Goheen, A.C., Cory, L. and Luhn, C. (1972). Annls. Phytopath. 23: 43.


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