Grapevine Fanleaf Virus (GFLV) - TAS ELISA                                 Home    Products    Testing Services   Supplies

Catalog #:  V215
Source Antibody:  A rabbit polyclonal antibody for capture and and a monoclonal antibody as detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test reacted with all GFLV isolates tested. Reaction of the ELISA is very strong.  Optical Density at 405nm is in a range of 0.300 - 3.000 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The virus can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.

Specificity:  There is no cross reaction found with healthy grapevine plant tissues.  Background is moderately low on the negative control wells.

Application:  The test can be used to detect GFLV in infected grapevine plant tissues.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V215-R1

ELISA Reagents, Alkaline Phosphatase

500

240

2 lb (1 kg)

V215-R2

ELISA Reagents, Alkaline Phosphatase

1000

415

3 lb (1.5 kg)

V215-R3

ELISA Reagents, Alkaline Phosphatase

5000

1660

12 lb (6 kg)
V215-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V215-K1 ELISA Kit, Alkaline Phosphatase

500

365

2 lb (1 kg)
V215-K2 ELISA Kit, Alkaline Phosphatase

1000

575

3 lb (1.5 kg)

V215-C1

Coating Antibody

500

120

2 lb (1 kg)

V215-C2

Coating Antibody

1000

215

2 lb (1 kg)

V215-C3

Coating Antibody

5000

750

2 lb (1 kg)

V215-D1

Detecting conjugate, Alkaline Phosphatase

500

155

2 lb (1 kg)

V215-D2

Detecting conjugate, Alkaline Phosphatase

1000

255

2 lb (1 kg)

V215-D3

Detecting conjugate, Alkaline Phosphatase

5000

960

2 lb (1 kg)
V215-P2 ELISA control, Positive

18

18

1 lb (0.5 kg)

V215-N1

Negative control

18

18

1 lb (0.5 kg)
M029-1 Buffers for DAS/TAS, for grape tests, alkaline phosphatase
500
100
2 lb (1.0 kg)
M029-2 Buffers for DAS/TAS, for grape tests, alkaline phosphatase
1000 
130
3 lb (1.5 kg)
M029-3 Buffers for DAS/TAS, for grape tests, alkaline phosphatase
5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instruction for TAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Grapevine Fanleaf Virus
Acronym:  GFLV
Synonyms:  Grapevine arriciamento virus, grapevine court-noue virus, grapevine infectious degeneration virus, grapevine Reisigkrankheit virus, grapevine roncet virus, grapevine urticado virus.
Group/Genus:  Nepoviruses

Vector:  Transmitted by a vector; a nematode; Xiphinema index (Hewitt et al., 1958) and X. italiae (Cohn et al., 1970). Virus does not require a helper virus for vector transmission.
Transmission:  transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; transmitted by seed (rarely if ever transmitted to embryos of Vitis vinifera, virus abundant in endosperm of infected viruses but not in embryo. Transmitted by seed in Chenopodium amaranticolor (Dias, 1963), C. quinoa (Brückbauer and Rüdel, 1961a), Glycine max (Cory and Hewitt, 1968); not transmitted by pollen (although found in pollen of Vitis).

Main host plants:  Grapevine, Vitis vinifera, V. rupestris and many other Vitis spp. and interspecific hybrids.
Virus Infection: cause symptoms of systemic green or yellow mosaic, ring and line patterns and flecks, leaf and nodal malformation. Symptoms persist and vary seasonally.

Diagnostically susceptible host species and symptoms: Vitis spp. - green or yellow systemic mosaic, rings, line patterns and flecks; Chenopodium amaranticolor and C. quinoa - systemic mottling and leaf malformation; Cucumis sativus, Phaseolus vulgaris cv. Bountiful - systemic chlorotic or necrotic mosaic, mottling, flecking or ringspots. Immune to some strains; Gomphrena globosa - systemic chlorotic spots, leaves twisted; Nicotiana benthamiana - systemic mottling, leaf malformation, stunting; Nicotiana clevelandii - systemic mottling and stunting.

Geographical distribution: Probably distributed worldwide (in all areas where Vitis vinifera and American hybrid rootstocks are grown).

Reference:
1. 
Hewitt, W.B., Martelli, G., Dias, H.F. and Taylor, R.H. (1970). CMI/AAB Descr. Pl. Viruses No. 28, 4 pp.
2. Quacquarelli, A., Gallitelli, D., Savino, V., and Martelli, G.P. (1976). J. gen. Virol. 32: 349.
3. Russo, M. (1985). Phytopathol. Medit. 24: 144


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