Grapevine Fanleaf Virus (GFLV) - TAS ELISA Home Products Testing Services Supplies
Catalog #: V215
Source Antibody: A rabbit polyclonal antibody for capture and and a
monoclonal antibody as detection.
Test Format: DAS
ELISA
Reactivity: This test reacted with all GFLV isolates tested. Reaction of the ELISA is very strong. Optical Density at 405nm is in a range of 9.300 - 3.000 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is very high. The virus can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.
Specificity: There is no cross reaction found with healthy grapevine plant tissues. Background is moderately low on the negative control wells.
Application: The test can be used to detect GFLV in infected grapevine plant tissues.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
|
V215-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
230 |
2 lb (1 kg) |
|
V215-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
395 |
3 lb (1.5 kg) |
|
V215-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1590 |
12 lb (6 kg) |
| V215-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
85 |
1 lb (0.5 kg) |
| V215-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
335 |
2 lb (1 kg) |
| V215-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
525 |
3 lb (1.5 kg) |
|
V215-C1 |
Coating Antibody |
500 |
115 |
2 lb (1 kg) |
|
V215-C2 |
Coating Antibody |
1000 |
205 |
2 lb (1 kg) |
|
V215-C3 |
Coating Antibody |
5000 |
725 |
2 lb (1 kg) |
|
V215-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
140 |
2 lb (1 kg) |
|
V215-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
225 |
2 lb (1 kg) |
|
V215-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
900 |
2 lb (1 kg) |
| V215-P2 | ELISA control, Positive |
18 |
15 |
1 lb (0.5 kg) |
|
V215-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
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An Order
To know more about the products: Product
Items
To learn how to perform the test: Instruction
for TAS ELISA Reagents
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Grapevine Fanleaf Virus
Acronym: GFLV
Synonyms: Grapevine arriciamento virus, grapevine court-noue virus,
grapevine infectious degeneration virus, grapevine Reisigkrankheit virus,
grapevine roncet virus, grapevine urticado virus.
Group/Genus: Nepoviruses
Vector: Transmitted by a vector; a nematode; Xiphinema index
(Hewitt et al., 1958) and X. italiae (Cohn et al., 1970).
Virus does not require a helper virus for vector transmission.
Transmission: transmitted by mechanical inoculation; transmitted by
grafting; not transmitted by contact between plants; transmitted by seed (rarely
if ever transmitted to embryos of Vitis vinifera, virus abundant in
endosperm of infected viruses but not in embryo. Transmitted by seed in
Chenopodium amaranticolor (Dias, 1963), C. quinoa (Brückbauer and
Rüdel, 1961a), Glycine max (Cory and Hewitt, 1968); not
transmitted by pollen (although found in pollen of Vitis).
Main host plants: Grapevine, Vitis vinifera, V. rupestris
and many other Vitis spp. and interspecific hybrids.
Virus Infection: cause symptoms of systemic green or yellow mosaic, ring
and line patterns and flecks, leaf and nodal malformation. Symptoms persist and
vary seasonally.
Diagnostically susceptible host species and symptoms: Vitis spp. - green or yellow systemic mosaic, rings, line patterns and flecks; Chenopodium amaranticolor and C. quinoa - systemic mottling and leaf malformation; Cucumis sativus, Phaseolus vulgaris cv. Bountiful - systemic chlorotic or necrotic mosaic, mottling, flecking or ringspots. Immune to some strains; Gomphrena globosa - systemic chlorotic spots, leaves twisted; Nicotiana benthamiana - systemic mottling, leaf malformation, stunting; Nicotiana clevelandii - systemic mottling and stunting.
Geographical distribution: Probably distributed worldwide (in all areas where Vitis vinifera and American hybrid rootstocks are grown).
Reference:
1. Hewitt, W.B., Martelli, G., Dias, H.F. and Taylor, R.H. (1970).
CMI/AAB Descr. Pl. Viruses No. 28, 4 pp.
2. Quacquarelli, A., Gallitelli, D., Savino, V., and Martelli, G.P.
(1976). J. gen. Virol. 32: 349.
3. Russo, M. (1985). Phytopathol. Medit. 24: 144