Catalog #: V027
Reactivity: Reaction of the ELISA is moderate strong. Optical Density at 405nm is in a range of 0.650 - 2.300 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is relatively high. The virus can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.
Specificity: There is no cross reaction found with healthy grapevine plant tissues. Background is moderately low on the negative control wells.
Application: The test can be used to detect GFLV in infected grapevine plant tissues.
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Information About the Virus
Name: Grapevine Fanleaf Virus
Vector: Transmitted by a vector; a nematode; Xiphinema index
(Hewitt et al., 1958) and X. italiae (Cohn et al., 1970).
Virus does not require a helper virus for vector transmission.
Main host plants: Grapevine, Vitis vinifera, V. rupestris
and many other Vitis spp. and interspecific hybrids.
Diagnostically susceptible host species and symptoms: Vitis spp. - green or yellow systemic mosaic, rings, line patterns and flecks; Chenopodium amaranticolor and C. quinoa - systemic mottling and leaf malformation; Cucumis sativus, Phaseolus vulgaris cv. Bountiful - systemic chlorotic or necrotic mosaic, mottling, flecking or ringspots. Immune to some strains; Gomphrena globosa - systemic chlorotic spots, leaves twisted; Nicotiana benthamiana - systemic mottling, leaf malformation, stunting; Nicotiana clevelandii - systemic mottling and stunting.
Geographical distribution: Probably distributed worldwide (in all areas where Vitis vinifera and American hybrid rootstocks are grown).
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