Grapevine Fanleaf Virus (GFLV) - DAS ELISA                                 Home    Products    Testing Services   Supplies

Catalog #:  V027
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  Reaction of the ELISA is moderate strong.  Optical Density at 405nm is in a range of 0.650 - 2.300 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.

Specificity:  There is no cross reaction found with healthy grapevine plant tissues.  Background is moderately low on the negative control wells.

Application:  The test can be used to detect GFLV in infected grapevine plant tissues.

Products: 

Catalog Number

Description

Test Wells

Price
  $US

Ship Weight
(Estimated)

V027-R1

ELISA Reagents, Alkaline Phosphatase

500

230

2 lb (1 kg)

V027-R2

ELISA Reagents, Alkaline Phosphatase

1000

395

3 lb (1.5 kg)

V027-R3

ELISA Reagents, Alkaline Phosphatase

5000

1590

12 lb (6 kg)
V027-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V027-K1 ELISA Kit, Alkaline Phosphatase

500

335

2 lb (1 kg)
V027-K2 ELISA Kit, Alkaline Phosphatase

1000

525

3 lb (1.5 kg)

V027-C1

Coating Antibody

500

115

2 lb (1 kg)

V027-C2

Coating Antibody

1000

205

2 lb (1 kg)

V027-C3

Coating Antibody

5000

725

2 lb (1 kg)

V027-D1

Detecting conjugate, Alkaline Phosphatase

500

140

2 lb (1 kg)

V027-D2

Detecting conjugate, Alkaline Phosphatase

1000

225

2 lb (1 kg)

V027-D3

Detecting conjugate, Alkaline Phosphatase

5000

900

2 lb (1 kg)
V027-P2 ELISA control, Positive

18

15

1 lb (0.5 kg)

V027-N1

Negative control

18

15

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500    90 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000     120 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000     350 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Grapevine Fanleaf Virus
Acronym:  GFLV
Synonyms:  Grapevine arriciamento virus, grapevine court-noue virus, grapevine infectious degeneration virus, grapevine Reisigkrankheit virus, grapevine roncet virus, grapevine urticado virus.
Group/Genus:  Nepoviruses

Vector:  Transmitted by a vector; a nematode; Xiphinema index (Hewitt et al., 1958) and X. italiae (Cohn et al., 1970). Virus does not require a helper virus for vector transmission.
Transmission:  transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; transmitted by seed (rarely if ever transmitted to embryos of Vitis vinifera, virus abundant in endosperm of infected viruses but not in embryo. Transmitted by seed in Chenopodium amaranticolor (Dias, 1963), C. quinoa (Brückbauer and Rüdel, 1961a), Glycine max (Cory and Hewitt, 1968); not transmitted by pollen (although found in pollen of Vitis).

Main host plants:  Grapevine, Vitis vinifera, V. rupestris and many other Vitis spp. and interspecific hybrids.
Virus Infection: cause symptoms of systemic green or yellow mosaic, ring and line patterns and flecks, leaf and nodal malformation. Symptoms persist and vary seasonally.

Diagnostically susceptible host species and symptoms: Vitis spp. - green or yellow systemic mosaic, rings, line patterns and flecks; Chenopodium amaranticolor and C. quinoa - systemic mottling and leaf malformation; Cucumis sativus, Phaseolus vulgaris cv. Bountiful - systemic chlorotic or necrotic mosaic, mottling, flecking or ringspots. Immune to some strains; Gomphrena globosa - systemic chlorotic spots, leaves twisted; Nicotiana benthamiana - systemic mottling, leaf malformation, stunting; Nicotiana clevelandii - systemic mottling and stunting.

Geographical distribution: Probably distributed worldwide (in all areas where Vitis vinifera and American hybrid rootstocks are grown).

Reference:
1. 
Hewitt, W.B., Martelli, G., Dias, H.F. and Taylor, R.H. (1970). CMI/AAB Descr. Pl. Viruses No. 28, 4 pp.
2. Quacquarelli, A., Gallitelli, D., Savino, V., and Martelli, G.P. (1976). J. gen. Virol. 32: 349.
3. Russo, M. (1985). Phytopathol. Medit. 24: 144


Home    Products    Testing Services   Supplies   Contact Us

nostics, plant pathogen detection, plant disease detection, plant virus detection, agri-diagnostics, tests for plant viruses, virus, plant bacterium, bacteria, ELISA tests for plant pathogens, Agdia, Plant diagnostics, plant diagnosis, Plant pathogen, plant pathogens, pathogen detection, laboratory services, ELISA Reagents, reagents, test kits, testing kit, antibody, antibodies, plant virus, plant viruses, virus detection, plant bacterium, plant bacteria, bacterial detection, fungus, fungi, fungal detection, testing services, virus disease, bacterial disease, plant diseases, symptoms, controls, plant clinic, plant seed, plant seeds, seed testing, seed pathogens, tomato diseases, ornamental diseases, potato diseases, seed pathogens, virus, viruses, AMV, ApMV, BCMV, BYDV, CMC, LMV, INSV, PLRV, PVA, PVM,PVS, PVX, PVY, POTY, PNYSV, SMV, SqMV, TAV, TMV, ToMV, TRSV, ToRSV, TSV, TSWV, TuMV, ZYMV, Plant pathogen detection, ELISA Reagents or ELISA kits for detection of plant pathogens, plant virus detection, plant bacterial detection, fungus detection, test services for plant pathogens, virus disease test, plant disease clinic, plant seed tests, seed test for pathogens, potato pathogen detection, tomato pathogen detection, ornamental pathogen detection, test for potato viruses, test for ornamental viruses, test for seed borne pathogens, plant bacteria, plant viruses, plant pathogens, plant disease, AC is a plant diagnostic company. AC Diagnostics provides ELISA Reagents and ELISA