Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Dogwood Mosaic Virus (DMV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V190
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all DMV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.800 - 2.700 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues such as rice and other host plants. Background is relative low on all of the negative control wells.
Application: The test can be used to detect DMV in infected host plants.
Products:
|
Catalog Number |
Description | Test Wells | Price $US |
Ship
Weight (Estimated) |
| V190-R1 | ELISA Reagents, Alkaline Phosphatase |
500 |
240 |
2 lb (1 kg) |
| V190-R2 | ELISA Reagents, Alkaline Phosphatase |
1000 |
415 |
3 lb (1.5 kg) |
| V190-R3 | ELISA Reagents, Alkaline Phosphatase |
5000 |
1660 |
12 lb (6 kg) |
| V190-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
95 |
1 lb (0.5 kg) |
| V190-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
355 |
2 lb (1 kg) |
| V190-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
565 |
3 lb (1.5 kg) |
| V190-C1 | Coating Antibody |
500 |
120 |
2 lb (1 kg) |
| V190-C2 | Coating Antibody |
1000 |
215 |
2 lb (1 kg) |
| V190-C3 | Coating Antibody |
5000 |
750 |
2 lb (1 kg) |
| V190-D1 | Detecting conjugate, Alkaline Phosphatase |
500 |
155 |
2 lb (1 kg) |
| V190-D2 | Detecting conjugate, Alkaline Phosphatase |
1000 |
255 |
2 lb (1 kg) |
| V190-D3 | Detecting conjugate, Alkaline Phosphatase |
5000 |
960 |
2 lb (1 kg) |
| V190-P2 | ELISA Control, Positive |
18 |
15 |
1 lb (0.5 kg) |
| V190-N1 | Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 |
3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 |
6 lb (3.0 kg) |
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An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Dogwood Mosaic Virus
Acronym: DMV
Synonyms: arabis mosaic virus (first considered to be a strain of this
virus, but now thought to be a separate viral species; Barnett, 1981; Barnett
and Baxter, 1976; Handley and Barnett, 1951; Reddick et al., 1980).
Group/Genus: Nepovirus
Vector: Not transmitted by Xiphinema diversicaudatum.
Transmission: Virus transmitted by mechanical inoculation; transmitted by
grafting.
Main host plants: Cornus florida
Virus Infection: Symptoms vary seasonally. Symptoms mosaic. Cornus
florida - symptomless or mottling or mosaic.
Diagnostically susceptible host species and symptoms: Chenopodium
amaranticolor - chlorotic local lesions, then systemic chlorotic spots, line
patterns, mosaic. C. quinoa - chlorotic local lesions becoming necrotic,
then systemic mosaic and tip necrosis. Vigna unguiculata ssp.
unguiculata, Lupinus albus - chlorotic ringspots and systemic mosaic.
Pisum sativum - chlorotic local lesions, then systemic mosaic. Torenia
fournieri - systemic chlorosis and veinal necrosis. Nicotiana
occidentalis - local lesions; systemic mosaic. N. tabacum - chlorotic
local lesions, then systemic in some cultivars. Petunia × hybrida -
necrotic ringspots; systemic necrotic spots. Lactuca sativa - systemic
mosaic.
References:
1. Barnett, O.W., Reddick, B.B., Burrows, P.M. and Baxter, L.W. (1989).
Phytopathology 79: 951.
2. Handley, M.K. and Barnett, O.W. (1981). Phytopathology 71:
223.