Dioscorea Latent Potexvirus (DLV) - DAS ELISA                      Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V229
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all DLV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.820 - 2.580 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted at 1:270-810.

Specificity:  There is no cross reaction with healthy plant  tissues. Background is relative low on all of  the negative control wells.

Application:  The test can be used to detect DLV in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)
V229-R1 ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)
V229-R2 ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)
V229-R3 ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V229-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V229-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V229-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)
V229-C1 Coating Antibody

500

125

2 lb (1 kg)
V229-C2 Coating Antibody

1000

235

2 lb (1 kg)
V229-C3 Coating Antibody

5000

805

2 lb (1 kg)
V229-D1 Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)
V229-D2 Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)
V229-D3 Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
V229-P1 ELISA Control, Positive

18

18

1 lb (0.5 kg)
V229-N1 Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Dioscorea Latent Potexvirus
Acronym:  DLV
Synonyms: 
Viral Family: Alphaflexiviridae

Vector: 
Transmission: Virus transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.
Main natural host plants: Dioscorea composita, and D. floribunda.

Virus Infection: Causes symptomless.
Diagnostically susceptible host species and symptoms:
Nicotiana megalosiphon, N. benthamiana - symptomless local and systemic infection; Philodendron speciosum, Phaseolus vulgaris cv. The Prince, Vigna unguiculata cv. Blackeye, Datura metel, D. stramonium, Nicotiana clevelandii, N. sylvestris, N. tabacum cv. Dutch A - local symptomless infection only.

References:
1. 
Hearon, S.S., Corbett, M.K., Lawson, R.H., Gillespie, A.G. and Waterworth, H.E. (1978). Phytopathology 68: 1137.
2.  Phillips, S. and Brunt, A.A. (1988). CMI/AAB Descr. Pl. Viruses No. 335, 2 pp.


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