Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Dioscorea Latent Potexvirus (DLV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V229
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all DLV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.820 - 2.580 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:270-810.
Specificity: There is no cross reaction with healthy plant tissues. Background is relative low on all of the negative control wells.
Application: The test can be used to detect DLV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
| V229-R1 | ELISA Reagents, Alkaline Phosphatase |
500 |
240 |
2 lb (1 kg) |
| V229-R2 | ELISA Reagents, Alkaline Phosphatase |
1000 |
415 |
3 lb (1.5 kg) |
| V229-R3 | ELISA Reagents, Alkaline Phosphatase |
5000 |
1660 |
12 lb (6 kg) |
| V229-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
95 |
1 lb (0.5 kg) |
| V229-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
355 |
2 lb (1 kg) |
| V229-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
565 |
3 lb (1.5 kg) |
| V229-C1 | Coating Antibody |
500 |
120 |
2 lb (1 kg) |
| V229-C2 | Coating Antibody |
1000 |
215 |
2 lb (1 kg) |
| V229-C3 | Coating Antibody |
5000 |
750 |
2 lb (1 kg) |
| V229-D1 | Detecting conjugate, Alkaline Phosphatase |
500 |
155 |
2 lb (1 kg) |
| V229-D2 | Detecting conjugate, Alkaline Phosphatase |
1000 |
255 |
2 lb (1 kg) |
| V229-D3 | Detecting conjugate, Alkaline Phosphatase |
5000 |
960 |
2 lb (1 kg) |
| V229-P1 | ELISA Control, Positive |
18 |
15 |
1 lb (0.5 kg) |
| V229-N1 | Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
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To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Dioscorea Latent Potexvirus
Acronym: DLV
Synonyms:
Viral Family: Alphaflexiviridae
Vector:
Transmission: Virus transmitted by mechanical inoculation; not
transmitted by contact between plants; not transmitted by seed; not transmitted
by pollen.
Main natural host plants: Dioscorea composita, and D.
floribunda.
Virus Infection: Causes symptomless.
Diagnostically susceptible host species and symptoms: Nicotiana
megalosiphon, N. benthamiana - symptomless local and systemic infection;
Philodendron speciosum, Phaseolus vulgaris cv. The Prince, Vigna
unguiculata cv. Blackeye, Datura metel, D. stramonium, Nicotiana
clevelandii, N. sylvestris, N. tabacum cv. Dutch A - local symptomless
infection only.
References:
1. Hearon, S.S., Corbett, M.K., Lawson, R.H., Gillespie, A.G. and
Waterworth, H.E. (1978). Phytopathology 68: 1137.
2. Phillips, S. and Brunt, A.A. (1988). CMI/AAB Descr. Pl. Viruses
No. 335, 2 pp.