Clavibacter michiganensis subsp. sepedonicus (Cms) - TAS ELISA        Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  B134
Source Antibody:  A rabbit polyclonal antibody for capture and and a monoclonal antibody as detection.
Test Format:  TAS ELISA                                   

Bacterial Ringrot: Bacterial ringrot is a severe disease of potato. Clavibacter michiganensis subsp. sepedonicus (Cms) is the pathogen of potato ringrot and has been reported in all major potato grow areas.

Reactivity:  This test system reacted with all Cms isolates tested.  Reaction of the ELISA is very strong.  Optical Density at 405nm  is in a range of 1.500 - 3.500 depending  on the bacterial titers in the samples to be tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The potato ringrot bacterium can be consistently detected in infected plant leaf tissues diluted at 1:810 - 1:2430.

Specificity:  This test system reacts negatively with Acidovorax avenae subsp. citrulli, Clavibacter flaccumfaciens, Clavibacter michiganensis subsp. insidiosus, C. m. subsp. michiganensis, C. m. subsp. nebraskensis, C. m. subsp. tessellarius, Erwinia carotovora subsp. atroseptica, E. c. subsp. carotovora, E. chrysanthemi, E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv. syringae, Ralstonia solanacearum, Xanthomonas campestris pv. armoraciae, X. c. pv. campestris, X. c. pv. citri, X. c. pv. diffenbachiae,  X. c. pv. pelargonii, X. c. pv. phaseoli, and X. c. pv. vesicatoria. 
There is no cross reaction with healthy plant tissues.  Background is low on all of the negative control wells.

Application:  The test can be used to detect Cms,  pathogen of potato ringrot, in infected host plants as well as in pure bacterial cultures.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

B134-R1

ELISA Reagents, Alkaline Phosphatase

500

230

2 lb (1 kg)

B134-R2

ELISA Reagents, Alkaline Phosphatase

1000

395

3 lb (1.5 kg)

B134-R3

ELISA Reagents, Alkaline Phosphatase

5000

1590

12 lb (6 kg)
B134-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
B134-K1 ELISA Kit, Alkaline Phosphatase

500

345

2 lb (1 kg)
B134-K2 ELISA Kit, Alkaline Phosphatase

1000

535

3 lb (1.5 kg)

B134-C1

Coating Antibody

500

115

2 lb (1 kg)

B134-C2

Coating Antibody

1000

205

2 lb (1 kg)

B134-C3

Coating Antibody

5000

725

2 lb (1 kg)

B134-D1

Detecting conjugate, Alkaline Phosphatase

500

140

2 lb (1 kg)

B134-D2

Detecting conjugate, Alkaline Phosphatase

1000

225

2 lb (1 kg)

B134-D3

Detecting conjugate, Alkaline Phosphatase

5000

900

2 lb (1 kg)

B134-P1

Positive control

18

20

1 lb (0.5 kg)

B134-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000  130 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000  400 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instruction for TAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls

Information About the Bacterium

Name:  Clavibacter michiganensis subsp. sepedonicus.
Acronym:  Cms
Synonyms: 
Group/Genus:  Clavibacter

Host plants and Diseases:  Bacterial ringrot of potato, 
Symptoms:  

Bacterial Transmission:  Seed tubes, infected plant residues, infected stems in soil, agricultural equipments.
Bacterial Infection:  Through wounds and natural openings such as stomata and trichomes.

Reference:  


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