Clavibacter michiganensis subsp. sepedonicus (Cms) - TAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: B134
Source Antibody: A rabbit polyclonal antibody for capture
and and a monoclonal antibody as detection.
Test Format: TAS ELISA
Bacterial Ringrot: Bacterial ringrot is a severe disease of potato. Clavibacter michiganensis subsp. sepedonicus (Cms) is the pathogen of potato ringrot and has been reported in all major potato grow areas.
Reactivity: This test system reacted with all Cms isolates tested. Reaction of the ELISA is very strong. Optical Density at 405nm is in a range of 1.500 - 3.500 depending on the bacterial titers in the samples to be tested.
Sensitivity: Sensitivity of the ELISA is very high. The potato ringrot bacterium can be consistently detected in infected plant leaf tissues diluted at 1:810 - 1:2430.
Specificity: This test system reacts
negatively with Acidovorax avenae subsp. citrulli, Clavibacter
flaccumfaciens, Clavibacter
michiganensis subsp. insidiosus, C. m. subsp. michiganensis, C. m. subsp. nebraskensis, C. m. subsp. tessellarius,
Erwinia carotovora subsp. atroseptica, E.
c. subsp. carotovora, E. chrysanthemi,
E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv.
syringae, Ralstonia solanacearum, Xanthomonas campestris pv.
armoraciae, X. c. pv. campestris, X. c. pv. citri, X. c.
pv. diffenbachiae, X. c. pv. pelargonii, X.
c. pv. phaseoli, and X. c. pv. vesicatoria.
There is no cross reaction with healthy plant
tissues. Background is low on all of the negative control wells.
Application: The test can be used to detect Cms, pathogen of potato ringrot, in infected host plants as well as in pure bacterial cultures.
Products:
|
Catalog Number |
Description | Test Wells | Price $US | Ship
Weight (Estimated) |
|
B134-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
220 |
2 lb (1 kg) |
|
B134-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
385 |
3 lb (1.5 kg) |
|
B134-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1515 |
12 lb (6 kg) |
| B134-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
85 |
1 lb (0.5 kg) |
| B134-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
330 |
2 lb (1 kg) |
| B134-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
520 |
3 lb (1.5 kg) |
|
B134-C1 |
Coating Antibody |
500 |
110 |
2 lb (1 kg) |
|
B134-C2 |
Coating Antibody |
1000 |
195 |
2 lb (1 kg) |
|
B134-C3 |
Coating Antibody |
5000 |
690 |
2 lb (1 kg) |
|
B134-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
135 |
2 lb (1 kg) |
|
B134-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
215 |
2 lb (1 kg) |
|
B134-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
860 |
2 lb (1 kg) |
|
B134-P1 |
Positive control |
18 |
20 |
1 lb (0.5 kg) |
|
B134-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
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An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Bacterium
Name: Clavibacter michiganensis subsp.
sepedonicus.
Acronym: Cms
Synonyms:
Group/Genus: Clavibacter
Host plants and Diseases: Bacterial ringrot of potato,
Symptoms:
Bacterial Transmission: Seed tubes, infected plant residues,
infected stems in soil, agricultural equipments.
Bacterial Infection: Through wounds and natural openings such
as stomata and trichomes.
Reference: