Clavibacter michiganensis subsp. sepedonicus (Cms) - IFA            Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  B135
Source Antibody:  A monoclonal antibody as detection.
Test Format:  Immunofluorescence Assay (IFA)                                   

Bacterial Ringrot: Bacterial ringrot is a severe disease of potato. Clavibacter michiganensis subsp. sepedonicus (Cms) is the pathogen of potato ringrot and has been reported in all major potato grow areas.

Reactivity:  This Immunofluorescence Assay (IFA) reacted with all Cms isolates tested.  This IFA procedure requires about 3 hours to perform.  Resulted FITC-fluorescenced bacteria on the slides can be visualized by fluorescence microscopy. 

Sensitivity:  The IFA test is very sensitive and accurate. The potato ringrot bacterium (Cms) can be consistently recognized among other contaminated bacteria and among plant tissues.

Specificity:  This test system reacts negatively with Acidovorax avenae subsp. citrulli, Clavibacter flaccumfaciens, Clavibacter michiganensis subsp. insidiosus, C. m. subsp. michiganensis, C. m. subsp. nebraskensis, C. m. subsp. tessellarius, Erwinia carotovora subsp. atroseptica, E. c. subsp. carotovora, E. chrysanthemi, E. stewartii, Pseudomonas syringae pv. phaseolicola, P. s. pv. syringae, Ralstonia solanacearum, Xanthomonas campestris pv. armoraciae, X. c. pv. campestris, X. c. pv. citri, X. c. pv. diffenbachiae,  X. c. pv. pelargonii, X. c. pv. phaseoli, and X. c. pv. vesicatoria. 

There is no cross reaction with healthy plant tissues.  Background is good under fluorescence microscopy views.

Application:  The test can be used to detect Cms,  pathogen of potato ringrot, in infected host plants as well as in pure bacterial cultures.

Products: 

Catalog Number

Description Test Wells Price $US Ship Weight
(Estimated)

B135-IF1

Immunofluorescence Assay (IFA) - FITC

50

75

1 lb (0.5 kg)

B135-IF2

Immunofluorescence Assay (IFA) - FITC

100

130

1 lb (0.5 kg)

B135-IF3

Immunofluorescence Assay (IFA) - FITC

250

220

1 lb (0.5 kg)

B135-IF4

Immunofluorescence Assay (IFA) - FITC

500

280

1 lb (0.5 kg)

B135-IF5

Immunofluorescence Assay (IFA) - FITC

1000

420

1 lb (0.5 kg)

B135-P1

Positive control

18

20

1 lb (0.5 kg)

B135-N1

Negative control

18

15

1 lb (0.5 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
Instruction In PDF FormatIn PUB Format
To be familiar with other components: Buffer Sets - PDF PUB; Controls - PDF PUB

Information About the Bacterium

Name:  Clavibacter michiganensis subsp. sepedonicus.
Acronym:  Cms
Synonyms: 
Group/Genus:  Clavibacter

Host plants and Diseases:  Bacterial ringrot of potato, 
Symptoms:  

Bacterial Transmission:  Seed tubes, infected plant residues, infected stems in soil, agricultural equipments.
Bacterial Infection:  Through wounds and natural openings such as stomata and trichomes.

Reference:  


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