Citrus Leaf Rugose Virus (CiLRV) - DAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V156
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all CiLRV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.800 - >2.500 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as citrus or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect CiLRV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V156-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V156-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V156-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V156-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V156-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V156-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V156-C1

Coating Antibody

500

125

2 lb (1 kg)

V156-C2

Coating Antibody

1000

235

2 lb (1 kg)

V156-C3

Coating Antibody

5000

805

2 lb (1 kg)

V156-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V156-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V156-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V156-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V156-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELIAS
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Citrus Leaf Rugose Virus
Acronym:  CiLRV
Synonyms:  citrus psorosis virus complex, citrus crinkly leaf ilarvirus
Group/Genus:  Ilarvirus

Vector:  Transmitted by means not involving a vector.
Transmission: Virus transmitted by mechanical inoculation; transmitted by grafting; possibly transmitted by seed.
Main natural host plants: Citrus aurantifolia, C. limon, C. paradisi

Virus Infection:  causes flecking of young leaves and malformation of old leaves (particularly of Eureka lemons), fruit small and lumpy. Symptoms persist, or vary seasonally.
Diagnostically susceptible host species and symptoms: Citrus limon Eureka lemon - asteroid local lesions. Citrus sinensis, C. limon, C. reticulata - crinkling and flecking. Vigna unguiculata - vein clearing, malformation. Crotalaria spectabilis - systemic chlorotic veinbanding.

Geographical distribution: Spreads in the USA (California). Found, but with no evidence of spread, in Australia.

References:
1. 
Attathom, S., Weathers, L.G. and Greer, F.C. (1975). Proc. Am. Phytopath. Soc. 2: 111.
2.  Gonsalves, D. and Garnsey, S.M. (1976). CMI/AAB Descr. Pl. Viruses No. 164, 4pp.


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