Cereal  Chlorotic Mottle Virus (CerCMV) - DAS ELISA            Home    Products    Testing Services   Supplies   Contact Us

Catalog #:
  V169
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all CerCMV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.650 - >2.850 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as cereal corps or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect CerCMV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V169-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V169-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V169-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V169-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V169-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V169-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V169-C1

Coating Antibody

500

125

2 lb (1 kg)

V169-C2

Coating Antibody

1000

235

2 lb (1 kg)

V169-C3

Coating Antibody

5000

805

2 lb (1 kg)

V169-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V169-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V169-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V169-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V169-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name: Cereal Chlorotic Mottle Virus
Acronym:  CerCMV
Synonyms:  
Group/Genus:  Nucleorhabdovirus

Vector: Transmitted by a vector; an insect; Nesoclutha pallida, Cicadula bimaculata, Cicadulina bipunctata ssp. bipunctella; Cicadellidae. Transmitted in a persistent manner. Virus retained when the vector moults; multiplies in the vector; not transmitted congenitally to the progeny of the vector.
Transmission:
  Virus not transmitted by mechanical inoculation; not transmitted by seed.
Main natural host plants: Avena sativa, Digitaria ciliaris, Dinebra retroflexa, Echinochloa colona, Hordeum vulgare, Leptochloa filiformis, Setaria verticillata, Triticum aestivum, Urochloa panicoides, Zea mays.

Virus Infection:  Symptoms include hlorotic streaking, variable stunting and sterility. Symptoms persist.
Diagnostically susceptible host species and symptoms: Dinebra retroflexa - chlorotic streaking; Leptochloa filiformis - chlorotic and necrotic streaking; Triticum spp. and Triticosecale - chlorotic streaking; Zea mays - chlorotic streaks, but cvs differ in susceptibility.

Geographical distribution: Spreads in Australia and Morocco.

References:
1. 
Greber, R.S. (1982). CMI/AAB Descr. Pl. Viruses No. 251, 4 pp.
2.  Lockhart, B.E.L. (1986). Plant Dis. 70: 912.


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