Celery Mosaic Virus (CeMV) - DAS ELISA                              Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V168
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all CeMV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405_nm  is in arrange of 0.850 - >3.000 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as celery or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect CeMV in infected host plants.

Products: 

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To know more about the products:  Product Items
To learn how to perform the test:  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name: Celery Mosaic Virus
Acronym:  CeMV
Synonyms:  apium virus 1, celery western mosaic virus, celery ringspot virus (Hollings, 1964; Pemberton and Frost, 1986).
Group/Genus:  Potyvirus

Vector: Transmitted by a vector; an insect; Myzus persicae, Aphis middletonii, A. ferruginea-striata; A. apigraveolens, A. apii, A. gossypii, A. rumicis, Cavariella capreae, Myzus circumflexus, M. convolvuli, Rhopalosiphum melliferum; Cavariella aegopodii, C. pastinacae (Severin and Freitag, 1968; Walkey et al., 1970); Aphididae. Transmitted in a non-persistent manner.
Transmission:  Virus transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed.
Main natural host plants: Apium graveolens var. dulce, Conium maculatum, Pastinaca sativa, Petroselinum crispum, Coriandrum sativum - chlorosis, stunted, Daucus carota - mosaic, Anethum graveolens.

Virus Infection:  Symptoms include systemic mottling, malformation of leaflets, crinkly leaves, chlorosis and necrotic spots, stunted and malformed. Symptoms persist.
Diagnostically susceptible host species and symptoms: Apium graveolens var. dulce - mottled and malformed leaflets; Conium maculatum - mottled and leaves malformed (Sutabutra, 1968); Pastinaca sativa - leaves malformed; Daucus carota ssp. sativus chlorotic spots; Coriandrum sativum - systemic chlorosis.

Geographical distribution: Probably distributed worldwide. Spreads in Argentina (Gracia and Feldman, 1977), Australia (western Australia), Canada (Kemp and Froud, 1975), the former Czechoslovakia (Chod, 1984)), France (Marchoux et al., 1969), Germany (Brandes and Luisoni, 1966), Italy (Avgelis and Quacquarelli, 1972), Japan (Iwaki and Komuro, 1970), New Zealand (Fry and Proctor, 1968), the UK (Walkey et al., 1970), the USA (in the western states and Florida (Purcifull and Shepard, 1967)).

References:
1.  Pemberton, A.W. and Frost, R.R. (1986). Ann. appl. Biol. 108: 319.
2.  Shepard, J.F. and Grogan, R.G. (1971). CMI/AAB Descr. Pl. Viruses No. 50, 3 pp.

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