Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Cactus X Virus (CVX) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V188
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all CVX isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.850 - >3.000 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is relatively high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
Specificity: There is no cross reaction with healthy plant tissues such as cactus or other host plants. Background is low on all of the negative control wells.
Application: The test can be used to detect CVX in infected host plants.
Products:
|
Catalog Number |
Description | Test Wells | Price $US |
Ship
Weight (Estimated) |
| V188-R1 | ELISA Reagents, Alkaline Phosphatase |
500 |
240 |
2 lb (1 kg) |
| V188-R2 | ELISA Reagents, Alkaline Phosphatase |
1000 |
415 |
3 lb (1.5 kg) |
| V188-R3 | ELISA Reagents, Alkaline Phosphatase |
5000 |
1660 |
12 lb (6 kg) |
| V188-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
95 |
1 lb (0.5 kg) |
| V188-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
355 |
2 lb (1 kg) |
| V188-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
565 |
3 lb (1.5 kg) |
| V188-C1 | Coating Antibody |
500 |
120 |
2 lb (1 kg) |
| V188-C2 | Coating Antibody |
1000 |
215 |
2 lb (1 kg) |
| V188-C3 | Coating Antibody |
5000 |
750 |
2 lb (1 kg) |
| V188-D1 | Detecting conjugate, Alkaline Phosphatase |
500 |
155 |
2 lb (1 kg) |
| V188-D2 | Detecting conjugate, Alkaline Phosphatase |
1000 |
255 |
2 lb (1 kg) |
| V188-D3 | Detecting conjugate, Alkaline Phosphatase |
5000 |
960 |
2 lb (1 kg) |
| V188-P2 | ELISA control, positive |
18 |
15 |
1 lb (0.5 kg) |
| V188-N1 | Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 |
3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 |
6 lb (3.0 kg) |
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To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Cactus X Virus
Acronym: CVX
Synonyms: barrel cactus virus (Attathom et al, 1978;
Milne, 1988).
Group/Genus: Potexvirus
Vector:
Transmission: Virus transmitted by mechanical inoculation; transmitted
by grafting; transmitted by contact between plants; not transmitted by seed.
Main natural host plants: Opuntia vulgaris,
Austrocylindropuntia cylindrica, Pereskia saccharosa, Schlumbergera bridgesii,
Epiphyllum spp., Cereus spp., Echinopsis spp., Zygocactus
spp., Ferocactus acanthodes, Echinocereus procumbens.
Virus Infection: Symptoms none. Usually infected symptomlessly.
Diagnostically susceptible host species and symptoms: Amaranthus
caudatus - necrotic local lesions; rarely systemic. Gomphrena globosa,
Chenopodium amaranticolor - necrotic local lesions; not systemic. C.
quinoa - systemic mottle.
References:
1. Milne, R.G. (1988). In: The Plant Viruses, Vol. 4; The
Filamentous Viruses, p.6; ed Milne, R.G., Plenum, New York.
2. Ready, K.F.M. and Bancroft, J.B. (1985). Virology 141:
302.