Citrus Tristeza Virus (CTV) - TAS ELISA                                         Home    Products    Testing Services   Supplies

Catalog #:  V216
Source Antibody:  A rabbit polyclonal antibody for capture and a monoclonal antibody as detection.
Test Format:  TAS ELISA                                   

Reactivity:  This test system reacted with all CTV virus isolates tested. Reaction of the ELISA is very strong.  Optical Density at 405nm  is in a range of 1.300 - >3.000 depending  on the virus titer in the samples tested.

Sensitivity:  This TAS-ELISA is a recently improved CTV tests with extreme sensitivity. This test is 3-4 time more sensitive than our original DAS-ELISA.  The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy citrus plant  tissues.  Background is very low on the negative control wells.

Application:  The test can be used to detect CTV in variously infected plant tissues of citrus.

Products: 

Catalog Number

Description Test Wells Price $US Ship Weight
(Estimated)

V216-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V216-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V216-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V216-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V216-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V216-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)
VS216-K1 DASI-ELISA kit based on 3DF1+3CA5 specific monoclonal antibodies

500

350

2 lb (1 kg)
VS216-K2 DASI-ELISA kit based on 3DF1+3CA5 specific monoclonal antibodies

1000

550

3 lb (1.5 kg)
VS216-K3 DASI-ELISA kit based on 3DF1+3CA5 specific monoclonal antibodies

5000

2030

12 lb (6 kg)

V216-C1

Coating Antibody

500

125

2 lb (1 kg)

V216-C2

Coating Antibody

1000

235

2 lb (1 kg)

V216-C3

Coating Antibody

5000

805

2 lb (1 kg)

V216-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V216-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V216-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V216-P1

Positive control

18

20

1 lb (0.5 kg)

V216-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instruction for TAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Citrus Tristeza Virus
Acronym:  CTV
Synonyms:  Citrus quick decline virus, grapefruit stem pitting virus, Hassaku dwarf virus, lime die-back virus, podredumbre de las raicillas. 
Group/Genus:  Closteroviruses

Vector:  Transmitted by a vector; an insect; Toxoptera citricidus (Meneghini, 1946; Costa and Grant, 1951), Aphis gossypii, A. spiraecola and Toxoptera aurantii (Dickson et al., 1951; Norman and Grant, 1956); Aphididae. Transmitted in a non-persistent manner.
Transmission:  Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by seed.
Main host plants:  Citrus, Citrus spp. grafted onto Citrus aurantifolia (sour orange) root stock, C. paradisi (grapefruit), C. aurantifolia (lime), C. aurantifolia (Seville orange), C. reticulata (mandarin), Aeglopsis chevalieri, Afraegle paniculata, Pamburus missionis, Passiflora gracilis.

Virus Infection:  Symptoms include quick decline, pitted stems, stunt die-back, decline and seedling yellows. Symptoms persist.
Diagnostically susceptible host species and symptoms: Citrus aurantifolia cvs West Indian lime, Key lime, Mexican lime, Galego lime, Kagzi lime - chlorotic vein flecks, leaves cupped or canoe-shaped; stem pitting (Wallace and Drake, 1951); C. sinensis on rootstock C. aurantifolia - sudden wilt, decline and death, overgrowth on bud union and `honeycombing' just below union; C. sinensis, C. paradisi - stem pitting.

Geographical distribution: Probably distributed worldwide (wherever citrus is grown). Spreads in the African region, the Eastern Asian region, the Eurasian region, the Mediterranean region, the North American region, and the Pacific region; Algeria, American Samoa, Antigua and Barbuda, Argentina, Australia, Belize, Bermuda, Bolivia, Brazil, Brunei Darussalam, Cameroon, the Central African Republic, Chad, China, Colombia, Costa Rica, Cyprus, the Dominican Republic, Ecuador, Egypt, El Salvador, Ethiopia, Fiji, French Polynesia, Gabon, Ghana, Guyana, India, Indonesia, Iran, Israel, Italy, Jamaica, Japan, Kenya, Korea Republic, Malaysia, Mauritius, Morocco, Mozambique, Nepal, Netherlands Antilles, New Caledonia, New Zealand, Nicaragua, Nigeria, Pakistan, Panama, Paraguay, Peru, the Philippines, Portugal, Puerto Rico, Saudi Arabia, Spain, Sri Lanka, Suriname, Taiwan, Tanzania, Thailand, Trinidad and Tobago, Turkey, the USA, Uganda, Uruguay, Venezuela, Vietnam, Zaire, Zambia, Western Samoa, the former Yugoslavia, Zimbabwe.

References:
1. 
Bar-Joseph, M. and Lee, R.F. (1989). CMI/AAB Descr. Pl. Viruses No. 353, 7 pp.
2. Brlansky, R.H., Lee, R.F. and Garnsey, S.M. (1988). Plant Dis. 72: 1039.
3. Lee, R.F., Calvert, L.A., Nagel, J. and Hubbard, J.D. (1988). Phytopathology 78: 1221


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