Citrus Tristeza Virus (CTV) - DAS ELISA                                         Home    Products    Testing Services   Supplies

Catalog #:  V018
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all virus isolates tested. Reaction of the ELISA is moderately strong.  Optical Density at 405nm  is in a range of 0.700 - 1.400 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant tissues diluted  at 1:270 - 1:810.

Specificity:  There is no obvious cross reaction with healthy citrus plant  tissues.  Background is relatively  low on the negative control wells.

Application:  The test can be used to detect CTV in infected citrus plants.

Products: 

Catalog Number

Description Test Wells Price $US Ship Weight
(Estimated)

V018-R1

ELISA Reagents, Alkaline Phosphatase

500

210

2 lb (1 kg)

V018-R2

ELISA Reagents, Alkaline Phosphatase

1000

370

3 lb (1.5 kg)

V018-R3

ELISA Reagents, Alkaline Phosphatase

5000

1450

12 lb (6 kg)
V018-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V018-K1 ELISA Kit, Alkaline Phosphatase

500

325

2 lb (1 kg)
V018-K2 ELISA Kit, Alkaline Phosphatase

1000

505

3 lb (1.5 kg)

V018-C1

Coating Antibody

500

105

2 lb (1 kg)

V018-C2

Coating Antibody

1000

185

2 lb (1 kg)

V018-C3

Coating Antibody

5000

660

2 lb (1 kg)

V018-D1

Detecting conjugate, Alkaline Phosphatase

500

130

2 lb (1 kg)

V018-D2

Detecting conjugate, Alkaline Phosphatase

1000

205

2 lb (1 kg)

V018-D3

Detecting conjugate, Alkaline Phosphatase

5000

820

2 lb (1 kg)
V018-LF1 Lateral flow device (Dip Strip)

10

65

2 lb (1 kg)
V018-LF2 Lateral flow device (Dip Strip)

25

105

3 lb (1.5 kg)
V018-LF3 Lateral flow device (Dip Strip)

100

310

5 lb (2.0 kg)

V018-P1

Positive control

18

15

1 lb (0.5 kg)

V018-N1

Negative control

18

15

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500    90 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000     120 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000     350 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Citrus Tristeza Virus
Acronym:  CTV
Synonyms:  Citrus quick decline virus, grapefruit stem pitting virus, Hassaku dwarf virus, lime die-back virus, podredumbre de las raicillas. 
Group/Genus:  Closteroviruses

Vector:  Transmitted by a vector; an insect; Toxoptera citricidus (Meneghini, 1946; Costa and Grant, 1951), Aphis gossypii, A. spiraecola and Toxoptera aurantii (Dickson et al., 1951; Norman and Grant, 1956); Aphididae. Transmitted in a non-persistent manner.
Transmission:  Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by seed.
Main host plants:  Citrus, Citrus spp. grafted onto Citrus aurantifolia (sour orange) root stock, C. paradisi (grapefruit), C. aurantifolia (lime), C. aurantifolia (Seville orange), C. reticulata (mandarin), Aeglopsis chevalieri, Afraegle paniculata, Pamburus missionis, Passiflora gracilis.

Virus Infection:  Symptoms include quick decline, pitted stems, stunt die-back, decline and seedling yellows. Symptoms persist.
Diagnostically susceptible host species and symptoms: Citrus aurantifolia cvs West Indian lime, Key lime, Mexican lime, Galego lime, Kagzi lime - chlorotic vein flecks, leaves cupped or canoe-shaped; stem pitting (Wallace and Drake, 1951); C. sinensis on rootstock C. aurantifolia - sudden wilt, decline and death, overgrowth on bud union and `honeycombing' just below union; C. sinensis, C. paradisi - stem pitting.

Geographical distribution: Probably distributed worldwide (wherever citrus is grown). Spreads in the African region, the Eastern Asian region, the Eurasian region, the Mediterranean region, the North American region, and the Pacific region; Algeria, American Samoa, Antigua and Barbuda, Argentina, Australia, Belize, Bermuda, Bolivia, Brazil, Brunei Darussalam, Cameroon, the Central African Republic, Chad, China, Colombia, Costa Rica, Cyprus, the Dominican Republic, Ecuador, Egypt, El Salvador, Ethiopia, Fiji, French Polynesia, Gabon, Ghana, Guyana, India, Indonesia, Iran, Israel, Italy, Jamaica, Japan, Kenya, Korea Republic, Malaysia, Mauritius, Morocco, Mozambique, Nepal, Netherlands Antilles, New Caledonia, New Zealand, Nicaragua, Nigeria, Pakistan, Panama, Paraguay, Peru, the Philippines, Portugal, Puerto Rico, Saudi Arabia, Spain, Sri Lanka, Suriname, Taiwan, Tanzania, Thailand, Trinidad and Tobago, Turkey, the USA, Uganda, Uruguay, Venezuela, Vietnam, Zaire, Zambia, Western Samoa, the former Yugoslavia, Zimbabwe.

References:
1. 
Bar-Joseph, M. and Lee, R.F. (1989). CMI/AAB Descr. Pl. Viruses No. 353, 7 pp.
2. Brlansky, R.H., Lee, R.F. and Garnsey, S.M. (1988). Plant Dis. 72: 1039.
3. Lee, R.F., Calvert, L.A., Nagel, J. and Hubbard, J.D. (1988). Phytopathology 78: 1221


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