Carrot Thin Leaf Virus (CTLV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V166
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all CTLV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.950 - >3.000 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
Specificity: There is no cross reaction with healthy plant tissues such as carrot or other host plants. Background is low on all of the negative control wells.
Application: The test can be used to detect CTLV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells | Price $US |
Ship
Weight (Estimated) |
|
V166-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
240 |
2 lb (1 kg) |
|
V166-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
415 |
3 lb (1.5 kg) |
|
V166-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1660 |
12 lb (6 kg) |
| V166-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
95 |
1 lb (0.5 kg) |
| V166-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
355 |
2 lb (1 kg) |
| V166-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
565 |
3 lb (1.5 kg) |
|
V166-C1 |
Coating Antibody |
500 |
120 |
2 lb (1 kg) |
|
V166-C2 |
Coating Antibody |
1000 |
215 |
2 lb (1 kg) |
|
V166-C3 |
Coating Antibody |
5000 |
750 |
2 lb (1 kg) |
|
V166-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
155 |
2 lb (1 kg) |
|
V166-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
255 |
2 lb (1 kg) |
|
V166-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
960 |
2 lb (1 kg) |
|
V166-P2 |
ELISA control, positive |
18 |
15 |
1 lb (0.5 kg) |
|
V166-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 |
3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 |
6 lb (3.0 kg) |
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To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Carrot Thin Leaf Virus
Acronym: CTLV
Synonyms:
Group/Genus: Potyvirus
Vector: Transmitted by a vector; an insect; Cavariella aegopodii,
Myzus persicae; Aphididae.
Transmission: Transmitted in a non-persistent manner. Virus
transmitted by mechanical inoculation; not transmitted by contact between
plants; not transmitted by seed.
Main natural host plants: Daucus carota, Conium maculatum, Daucus
carota ssp. sativus.
Virus Infection: Symptoms include leaflet lobes malformed and narrow,
vein clearing and some chlorotic spots, and and some vein clearing. Symptoms
persist.
Diagnostically susceptible host species and symptoms: Anthriscus
cerefolium - thread like leaflets; Apium australe - chlorosis and
death of older leaves; Chenopodium quinoa - chlorotic and necrotic local
lesions; C. amaranticolor - chlorotic and red ring local lesions;
Coriandrum sativum, Daucus carota ssp. sativus - thread like
leaflets, vein clearing; Pastinaca sativa, Petroselinum crispum, Zinnia
elegans - symptomless
Geographical distribution: Spreads in the USA.
References:
1. Howell, W.E. and Mink, G.I. (1976). Phytopathology 66:
949.
2. Howell, W.E. and Mink, G.I. (1980). CMI/AAB Descr. Pl.
Viruses No. 218, 3 pp