Carrot Thin Leaf Virus (CTLV) - DAS ELISA                              Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V166
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all CTLV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.950 - >3.000 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as carrot or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect CTLV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V166-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V166-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V166-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V166-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V166-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V166-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V166-C1

Coating Antibody

500

125

2 lb (1 kg)

V166-C2

Coating Antibody

1000

235

2 lb (1 kg)

V166-C3

Coating Antibody

5000

805

2 lb (1 kg)

V166-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V166-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V166-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V166-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V166-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name: Carrot Thin Leaf Virus
Acronym:  CTLV
Synonyms:  
Group/Genus:  Potyvirus

Vector: Transmitted by a vector; an insect; Cavariella aegopodii, Myzus persicae; Aphididae.
Transmission:
  Transmitted in a non-persistent manner. Virus transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed.
Main natural host plants: Daucus carota, Conium maculatum, Daucus carota ssp. sativus.

Virus Infection:  Symptoms include leaflet lobes malformed and narrow, vein clearing and some chlorotic spots, and and some vein clearing. Symptoms persist.
Diagnostically susceptible host species and symptoms: Anthriscus cerefolium - thread like leaflets; Apium australe - chlorosis and death of older leaves; Chenopodium quinoa - chlorotic and necrotic local lesions; C. amaranticolor - chlorotic and red ring local lesions; Coriandrum sativum, Daucus carota ssp. sativus - thread like leaflets, vein clearing; Pastinaca sativa, Petroselinum crispum, Zinnia elegans - symptomless

Geographical distribution: Spreads in the USA.

References:
1. 
Howell, W.E. and Mink, G.I. (1976). Phytopathology 66: 949.
2.  Howell, W.E. and Mink, G.I. (1980). CMI/AAB Descr. Pl. Viruses No. 218, 3 pp


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