Cowpea Mottle Virus (CPMoV) - DAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V151
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all CPMoV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.900 - >2.500 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as cowpea, pea, bean or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect CPMoV in infected pea, bean and other infected host plant tissues.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V151-R1

ELISA Reagents, Alkaline Phosphatase

500

240

2 lb (1 kg)

V151-R2

ELISA Reagents, Alkaline Phosphatase

1000

415

3 lb (1.5 kg)

V151-R3

ELISA Reagents, Alkaline Phosphatase

5000

1660

12 lb (6 kg)
V151-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V151-K1 ELISA Kit, Alkaline Phosphatase

500

365

2 lb (1 kg)
V151-K2 ELISA Kit, Alkaline Phosphatase

1000

575

3 lb (1.5 kg)

V151-C1

Coating Antibody

500

120

2 lb (1 kg)

V151-C2

Coating Antibody

1000

215

2 lb (1 kg)

V151-C3

Coating Antibody

5000

750

2 lb (1 kg)

V151-D1

Detecting conjugate, Alkaline Phosphatase

500

155

2 lb (1 kg)

V151-D2

Detecting conjugate, Alkaline Phosphatase

1000

255

2 lb (1 kg)

V151-D3

Detecting conjugate, Alkaline Phosphatase

5000

960

2 lb (1 kg)

V151-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V151-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500 100 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000  130 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000  400 6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Cowpea Mottle Virus
Acronym:  CPMoV
Synonyms:  alfalfa latent virus, Steinklee Virus, Wisconsin pea streak virus, probably pea streak New Zealand virus (Bos, 1964; Chamberlain, 1954).
Group/Genus:  Carmovirus

Vector:  Transmitted by a vector; an insect; Ootheca mutabilis, Paraluperodes lineata; Coleoptera.
Transmission: Virus transmitted by mechanical inoculation; transmitted by seed (10% in V. unguiculata).
Main natural host plants: Vigna unguiculata, Vigna subterranea.

Virus Infection:  Symptoms by viral infection include mottling or mosaic, green mottle, shortening of petioles. Symptoms persist.
Diagnostically susceptible host species and symptoms: Chenopodium amaranticolor, C. quinoa - diffuse chlorotic local lesions; not systemic; Cajanus cajan, Vigna unguiculata - severe systemic chlorotic or green mottle, leaf malformation. Necrosis in youngest primary leaves; Glycine max, Macroptilium lathyroides, Phaseolus acutifolius, P. lunatus, P. vulgaris, Vigna radiata, V. mungo - systemic mosaic.

Geographical distribution: Spreads in Nigeria.

References:
1. 
Bozarth, R.F. and Shoyinka, S.A. (1979). CMI/AAB Descr. Pl. Viruses No. 212, 3 pp.
2.  Kim, J.W. (1989). Ph.D. Thesis. Indiana State University, Terra Haute, Indiana, U.S.A.


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