Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Cucumber Necrosis Virus (CNV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V185
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all CNV isolates tested. Reaction of the ELISA is very strong. Optical Density at 405nm is in arrange of 1.100 - >3.000 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is very high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
Specificity: There is no cross reaction with healthy plant tissues such as cucumber and other host plants. Background is very low on all of the negative control wells.
Application: The test can be used to detect CNV in infected host plants.
Products:
|
Catalog Number |
Description | Test Wells | Price $US |
Ship
Weight (Estimated) |
| V185-R1 | ELISA Reagents, Alkaline Phosphatase |
500 |
250 |
2 lb (1 kg) |
| V185-R2 | ELISA Reagents, Alkaline Phosphatase |
1000 |
425 |
3 lb (1.5 kg) |
| V185-R3 | ELISA Reagents, Alkaline Phosphatase |
5000 |
1690 |
12 lb (6 kg) |
| V185-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
95 |
1 lb (0.5 kg) |
| V185-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
385 |
2 lb (1 kg) |
| V185-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
610 |
3 lb (1.5 kg) |
| V185-C1 | Coating Antibody |
500 |
125 |
2 lb (1 kg) |
| V185-C2 | Coating Antibody |
1000 |
235 |
2 lb (1 kg) |
| V185-C3 | Coating Antibody |
5000 |
805 |
2 lb (1 kg) |
| V185-D1 | Detecting conjugate, Alkaline Phosphatase |
500 |
170 |
2 lb (1 kg) |
| V185-D2 | Detecting conjugate, Alkaline Phosphatase |
1000 |
285 |
2 lb (1 kg) |
| V185-D3 | Detecting conjugate, Alkaline Phosphatase |
5000 |
980 |
2 lb (1 kg) |
| V185-P1 | Positive control |
18 |
15 |
1 lb (0.5 kg) |
| V185-N1 | Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 |
2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 |
3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 |
6 lb (3.0 kg) |
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An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Cucumber Necrosis Virus
Acronym: CNV
Synonyms:
Group/Genus: Tombusvirus
Vector: Transmitted by a vector; an insect; Sesselia pusilla,
Chaetocnema pulla, Trichispa serica and Dicladispa viridicyanea;
Coleoptera. Transmitted in a semi-persistent manner. Virus lost by the vector
when it moults; does not multiply in the vector; not transmitted congenitally to
the progeny of the vector; does not require a helper virus for vector
transmission.
Transmission: Virus transmitted by mechanical inoculation; transmitted by
contact between plants; not transmitted by seed; not transmitted by pollen.
Main host plants: Symptoms vary seasonally.Cucumis sativus
Virus Infection: Symptoms malformed chlorotic or purplish leaves,
which dessicate and die. Fruits mottled.
Diagnostically susceptible host species and symptoms: Nicotiana
clevelandii - necrotic local lesions, systemic necrosis. Nicotiana
benthamiana - systemic necrosis. Chenopodium amaranticolor - small
necrotic local lesions, no systemic infection. Vigna unguiculata - brown
necrotic local lesions, no systemic infection. Gomphrena globosa - grey
necrotic local lesions, no systemic infection. Nicotiana tabacum - grey
necrotic spots, no systemic infection.
References:
1. Rochon, D.M., Johnston, J.C. and Riviere, C.J. (1991). Can. J.
Pl. Pathol. 13: 162.
2. Riviere, C.J. and Rochon, D.M. (1990). J. gen. Virol. 71:
1887.