Cucumber Necrosis Virus (CNV) - DAS ELISA                            Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V185
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all CNV isolates tested. Reaction of the ELISA is very strong.  Optical Density at 405nm  is in arrange of 1.100 - >3.000 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as cucumber and other host plants. Background is very low on all of  the negative control wells.

Application:  The test can be used to detect CNV in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)
V185-R1 ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)
V185-R2 ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)
V185-R3 ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V185-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V185-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V185-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)
V185-C1 Coating Antibody

500

125

2 lb (1 kg)
V185-C2 Coating Antibody

1000

235

2 lb (1 kg)
V185-C3 Coating Antibody

5000

805

2 lb (1 kg)
V185-D1 Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)
V185-D2 Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)
V185-D3 Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
V185-P1 Positive control

18

15

1 lb (0.5 kg)
V185-N1 Negative control

18

15

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Cucumber Necrosis Virus
Acronym:  CNV
Synonyms:  
Group/Genus: Tombusvirus

Vector:  Transmitted by a vector; an insect; Sesselia pusilla, Chaetocnema pulla, Trichispa serica and Dicladispa viridicyanea; Coleoptera. Transmitted in a semi-persistent manner. Virus lost by the vector when it moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector; does not require a helper virus for vector transmission.
Transmission: Virus transmitted by mechanical inoculation; transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.
Main host plants:  Symptoms vary seasonally.Cucumis sativus

Virus Infection:  Symptoms malformed chlorotic or purplish leaves, which dessicate and die. Fruits mottled.
Diagnostically susceptible host species and symptoms:
Nicotiana clevelandii - necrotic local lesions, systemic necrosis. Nicotiana benthamiana - systemic necrosis. Chenopodium amaranticolor - small necrotic local lesions, no systemic infection. Vigna unguiculata - brown necrotic local lesions, no systemic infection. Gomphrena globosa - grey necrotic local lesions, no systemic infection. Nicotiana tabacum - grey necrotic spots, no systemic infection.

Geographical distribution: Spreads in Canada (Ontario).

 References:
1. 
Rochon, D.M., Johnston, J.C. and Riviere, C.J. (1991). Can. J. Pl. Pathol. 13: 162.
2. Riviere, C.J. and Rochon, D.M. (1990). J. gen. Virol. 71: 1887.


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