Bt Cry 1Ac Protein - DAS ELISA                                                Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  G131
Source Antibody:  A rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                              

Reactivity:   This ELISA detects Bt Cry1Ac proteins in expressed in transgenic host plants, such as corn and cotton. The test is successful in detecting Bt Cry1Ac protein in leaves, seedlings, and seeds of cotton and corn varieties.

Reaction of this test is strong.  Depending on the protein titers in the samples tested, optical Density at 405_nm  is in a range of 1.500 - 2.500 for corn leaves and 2.500 - >3.000 for cotton leaves.

Sensitivity:  Sensitivity of the ELISA is high.  This test can be used for quantitative detection of Bt Cry1Ac protein in plant tissues, if a series dilutions of the purified Bt Cry1Ac protein is used as standard curve..

Specificity:   This test shows no cross reaction with Bt Cry1Ab, Bt Cry1F, Bt Cry2A, Bt Cry3A, Bt Cry3Bb1, Bt Cry9C, or other Bt proteins.

There is no cross reaction with healthy plant tissues.  Background is low on all of the negative control wells.

Application:  The test can be used to detect the Bt Cry1Ac protein in transgenic host plant tissues such as leaves, seeds, seedlings, and roots qualitatively and quantitatively.

Products: 

It is easy and convenient To Place An Order
To know more about the products:  Product Items
To learn how to perform the test:  Instructions for DAS ELISA  In PDF Format  In PUB Format
To be familiar with other components: Buffer Sets - PDF PUB; Controls - PDF PUB

Information About the Bt Cry1Ac

Name:  Bacillus thuringiensis Cry1Ac protein
Acronym:  Bt Cry1Ac
Synonyms: 
Group/Genus: Bacillus

Reference:

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