Barley Mild Mosaic Virus (BaMMV) - DAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V141
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all BaMMV isolates tested. Reaction of the ELISA is very strong.  Optical Density at 405nm  is in arrange of 1.100 - >3.000 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as barley, corn or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect BaMMV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V141-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V141-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V141-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V141-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V141-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V141-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V141-C1

Coating Antibody

500

125

2 lb (1 kg)

V141-C2

Coating Antibody

1000

235

2 lb (1 kg)

V141-C3

Coating Antibody

5000

805

2 lb (1 kg)

V141-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V141-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V141-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V141-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V141-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Barley Mild Mosaic Virus
Acronym:  BaMMV
Synonyms:  barley yellow mosaic virus mechanically transmissible strain, BaYMV - Streatley strain
Group/Genus:  Bymovirus

Vector:  Transmitted by a vector; a fungus; Polymyxa graminis (Mastigomycotina); Plasmodiophorales. Principal natural vector(s): Polymyxa graminis.
Transmission: Virus transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.
Main natural host plants: Hordeum vulgare.

Virus Infection:  Symptoms vary seasonally, in winter or early spring only. Symptoms yellow streaks, brown necrosis. Leaves may roll making the plant look `spiky'.
Diagnostically susceptible host species and symptoms: Hordeum vulgare, Lagurus ovatus, Eremopyrum hirsutum, Aegilops kotschyi, A. searsii, A. speltoides - systemic leaf streaking.

Geographical distribution: Spreads in Belgium, France, Germany, Italy, Japan, the Netherlands, and the UK.

References:
1. 
Adams, M.J., Jones, P. and Swaby, A.G. (1987). Ann. appl. Biol. 110: 321.
2.  Huth, W. and Adams, M.J. (1990). Intervirology 31: 38.


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