Barley Mild Mosaic Virus (BaMMV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V141
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all BaMMV isolates tested. Reaction of the ELISA is very strong. Optical Density at 405nm is in arrange of 1.100 - >3.000 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
Specificity: There is no cross reaction with healthy plant tissues such as barley, corn or other host plants. Background is low on all of the negative control wells.
Application: The test can be used to detect BaMMV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
|
V141-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
230 |
2 lb (1 kg) |
|
V141-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
395 |
3 lb (1.5 kg) |
|
V141-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1590 |
12 lb (6 kg) |
| V141-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
85 |
1 lb (0.5 kg) |
| V141-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
335 |
2 lb (1 kg) |
| V141-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
525 |
3 lb (1.5 kg) |
|
V141-C1 |
Coating Antibody |
500 |
115 |
2 lb (1 kg) |
|
V141-C2 |
Coating Antibody |
1000 |
205 |
2 lb (1 kg) |
|
V141-C3 |
Coating Antibody |
5000 |
725 |
2 lb (1 kg) |
|
V141-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
140 |
2 lb (1 kg) |
|
V141-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
225 |
2 lb (1 kg) |
|
V141-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
900 |
2 lb (1 kg) |
|
V141-P2 |
ELISA control, positive |
18 |
15 |
1 lb (0.5 kg) |
|
V141-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
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To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Barley Mild Mosaic Virus
Acronym: BaMMV
Synonyms: barley yellow mosaic virus mechanically transmissible
strain, BaYMV - Streatley strain
Group/Genus: Bymovirus
Vector: Transmitted by a vector; a fungus; Polymyxa graminis (Mastigomycotina);
Plasmodiophorales. Principal natural vector(s): Polymyxa graminis.
Transmission: Virus transmitted by mechanical inoculation; not
transmitted by contact between plants; not transmitted by seed; not transmitted
by pollen.
Main natural host plants: Hordeum vulgare.
Virus Infection: Symptoms vary seasonally, in winter or
early spring only. Symptoms yellow streaks, brown necrosis. Leaves may roll
making the plant look `spiky'.
Diagnostically susceptible host species and symptoms: Hordeum vulgare,
Lagurus ovatus, Eremopyrum hirsutum, Aegilops kotschyi, A. searsii, A.
speltoides - systemic leaf streaking.
Geographical distribution: Spreads in Belgium, France, Germany, Italy, Japan, the Netherlands, and the UK.
References:
1. Adams, M.J., Jones, P. and Swaby, A.G. (1987). Ann. appl. Biol.
110: 321.
2. Huth, W. and Adams, M.J. (1990). Intervirology 31:
38.