Blueberry Shoestring Virus (BSSV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V152
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all BSSV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.600 - >2.300 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues such as blueberry, or other host plants. Background is relative low on all of the negative control wells.
Application: The test can be used to detect BSSV in infected host plant tissues.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
|
V152-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
210 |
2 lb (1 kg) |
|
V152-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
370 |
3 lb (1.5 kg) |
|
V152-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1450 |
12 lb (6 kg) |
| V152-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
85 |
1 lb (0.5 kg) |
| V152-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
325 |
2 lb (1 kg) |
| V152-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
505 |
3 lb (1.5 kg) |
|
V152-C1 |
Coating Antibody |
500 |
105 |
2 lb (1 kg) |
|
V152-C2 |
Coating Antibody |
1000 |
185 |
2 lb (1 kg) |
|
V152-C3 |
Coating Antibody |
5000 |
660 |
2 lb (1 kg) |
|
V152-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
130 |
2 lb (1 kg) |
|
V152-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
205 |
2 lb (1 kg) |
|
V152-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
820 |
2 lb (1 kg) |
|
V152-P2 |
ELISA control, positive |
18 |
15 |
1 lb (0.5 kg) |
|
V152-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 | 6 lb (3.0 kg) |
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Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Blueberry Shoestring Virus
Acronym: BSSV
Synonyms:
Group/Genus: Sobemovirus
Vector: Transmitted by a vector; an insect; Masonaphis pepperi;
Aphididae. Transmitted in a semi-persistent manner (probably). Virus not
transmitted congenitally to the progeny of the vector.
Transmission: Transmitted by mechanical inoculation; transmitted by
grafting; not transmitted by contact between plants; not transmitted by seed;
not transmitted by pollen.
Main natural host plants: Vaccinium corymbosum, V.
angustifolium.
Virus Infection: Symptoms by viral infection include
"shoestring" disease; reddish streaks on stems, narrow strap-like leaves,
occasional flower-breaking, severe yield reduction. Symptoms persist and vary
seasonally.
Diagnostically susceptible host species and symptoms: Vaccinium
spp. - oak leaf pattern on leaves malformed shoestring leaves, red streaks on
stems; blossoms pink.
Geographical distribution: Spreads in the USA (in Michigan and New Jersey).
References:
1. Converse, R.H., Martin, R.R. and Spiegel, S. (1987). In: Virus
Diseases of Small Fruits; ed. R.H. Converse. U.S. Dep. Agric. Hdbk No. 631.
2. Ramsdell, D.C. (1979a). CMI/AAB Descr. Pl. Viruses
No. 204, 4 pp.