Blueberry Shoestring Virus (BSSV) - DAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V152
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all BSSV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.600 - >2.300 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as blueberry, or other host plants. Background is relative low on all of  the negative control wells.

Application:  The test can be used to detect BSSV in infected host plant tissues.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V152-R1

ELISA Reagents, Alkaline Phosphatase

500

240

2 lb (1 kg)

V152-R2

ELISA Reagents, Alkaline Phosphatase

1000

415

3 lb (1.5 kg)

V152-R3

ELISA Reagents, Alkaline Phosphatase

5000

1660

12 lb (6 kg)
V152-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V152-K1 ELISA Kit, Alkaline Phosphatase

500

365

2 lb (1 kg)
V152-K2 ELISA Kit, Alkaline Phosphatase

1000

575

3 lb (1.5 kg)

V152-C1

Coating Antibody

500

120

2 lb (1 kg)

V152-C2

Coating Antibody

1000

215

2 lb (1 kg)

V152-C3

Coating Antibody

5000

750

2 lb (1 kg)

V152-D1

Detecting conjugate, Alkaline Phosphatase

500

155

2 lb (1 kg)

V152-D2

Detecting conjugate, Alkaline Phosphatase

1000

255

2 lb (1 kg)

V152-D3

Detecting conjugate, Alkaline Phosphatase

5000

960

2 lb (1 kg)

V152-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V152-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Blueberry Shoestring Virus
Acronym:  BSSV
Synonyms:  
Group/Genus:  Sobemovirus

Vector:  Transmitted by a vector; an insect; Masonaphis pepperi; Aphididae. Transmitted in a semi-persistent manner (probably). Virus not transmitted congenitally to the progeny of the vector.
Transmission: Transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.
Main natural host plants: Vaccinium corymbosum, V. angustifolium.

Virus Infection:  Symptoms by viral infection include "shoestring" disease; reddish streaks on stems, narrow strap-like leaves, occasional flower-breaking, severe yield reduction. Symptoms persist and vary seasonally.
Diagnostically susceptible host species and symptoms: Vaccinium spp. - oak leaf pattern on leaves malformed shoestring leaves, red streaks on stems; blossoms pink.

Geographical distribution: Spreads in the USA (in Michigan and New Jersey).

References:
1. 
Converse, R.H., Martin, R.R. and Spiegel, S. (1987). In: Virus Diseases of Small Fruits; ed. R.H. Converse. U.S. Dep. Agric. Hdbk No. 631.
2.  Ramsdell, D.C. (1979a). CMI/AAB Descr. Pl. Viruses No. 204, 4 pp.


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