Barley Stripe Mosaic Virus (BSMV) - DAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V005
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacts with all known strains of the virus.  Reaction of the ELISA is relatively strong.  Optical Density at 405nm is in a range of 1.480 - 2.200 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The virus can be consistently detected in infected plant tissues diluted at 1:2430.

Specificity:    There is no obvious cross reaction with healthy plant tissues such as barley and wheat. Background is moderate  low on the negative control wells.

Application:  The test can be used to detect BSMV in infected wheat and barley.

Products: 

Catalog Number

Description Test Wells Price $US Ship Weight
(Estimated)
V005-R1 ELISA Reagents, Alkaline Phosphatase

500

210

2 lb (1 kg)
V005-R2 ELISA Reagents, Alkaline Phosphatase

1000

370

3 lb (1.5 kg)
V005-R3 ELISA Reagents, Alkaline Phosphatase

5000

1450

12 lb (6 kg)
V005-R4 ELISA Reagents, Alkaline Phosphatase

100

85

1 lb (0.5 kg)
V005-K1 ELISA Kit, Alkaline Phosphatase

500

325

2 lb (1 kg)
V005-K2 ELISA Kit, Alkaline Phosphatase

1000

505

3 lb (1.5 kg)
V005-C1 Coating Antibody

500

105

2 lb (1 kg)
V005-C2 Coating Antibody

1000

185

2 lb (1 kg)
V005-C3 Coating Antibody

5000

660

2 lb (1 kg)
V005-D1 Detecting conjugate, Alkaline Phosphatase

500

130

2 lb (1 kg)
V005-D2 Detecting conjugate, Alkaline Phosphatase

1000

205

2 lb (1 kg)
V005-D3 Detecting conjugate, Alkaline Phosphatase

5000

820

2 lb (1 kg)
V005-P1 Positive control

18

18

1 lb (0.5 kg)
V005-N1 Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500
90
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 
120
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase
5000 
350
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
   Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Barley Stripe Mosaic Virus
Acronym:  BSMV
Synonyms:   barley false stripe virus, possibly barley yellow stripe virus, barley mild stripe virus, oat stripe mosaic virus.
Group/Genus:  Hordeiviruses

Vector:  Transmitted by means not involving a vector.
Transmission:  Virus transmitted by mechanical inoculation; transmitted by seed (up to 90-100%); transmitted by pollen to the pollinated plant.
Main host plants:  Hordeum vulgare, Triticum aestivum  

Virus Infection:  Symptoms persist. Mild stripe mosaic to lethal necrosis.
Diagnostically susceptible host species and symptoms: Hordeum vulgare, Triticum aestivum, Avena sativa - systemic stripe mosaic. Chenopodium amaranticolor, C. quinoa, C. album - large chlorotic local lesions; not systemic. Beta vulgaris - chlorotic local lesions; not systemic. Zea mays - systemic stripe mosaic. Spinacia oleracea - systemic mosaic. Nicotiana tabacum cv. Samsun - local chlorotic lesions; not systemic.

Geographical distribution: Probably distributed worldwide. Spreads in the Eurasian region, the North American region, and the Pacific region; Australia, China, the UK, the USA, and the former USSR.

References:
1. 
Edwards, M.C., Petty, I.T.D. and Jackson, A.O. (1992). Virology 189: 389.
2.  Jackson, A.O., Petty, I.T.D., Jones, R.W., Edwards, M.C. and French, R. (1991). Semin. Virol. 2: 107.


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