Bean Rugose Mosaic Virus (BRMV) - DAS ELISA                         Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V124
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all virus isolates tested.  Reaction of the ELISA is moderate.  Optical Density at 405nm  is in arrange of 0.800 - 1.200 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no obvious cross reaction with healthy plant  tissues.  Background is low on the negative control wells including healthy plant sample and buffer.

Application:  The test can be used to detect BRMV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V124-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V124-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V124-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V124-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V124-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V124-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V124-C1

Coating Antibody

500

125

2 lb (1 kg)

V124-C2

Coating Antibody

1000

235

2 lb (1 kg)

V124-C3

Coating Antibody

5000

805

2 lb (1 kg)

V124-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V124-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V124-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
V124-P2 ELISA control, Positive

18

18

1 lb (0.5 kg)

V124-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELIAS
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Bean Rugose Mosaic Virus
Acronym:  BRMV
Synonyms:  
Group/Genus:  Comoviruses

Vector:  a vector; an insect; Ceratoma ruficornis, Diabrotica balteata, D. adelpha; Coleoptera
Transmission: mechanical transmission, but not by seeds.
Main host plants:  Phaseolus vulgaris

Virus Infection:  cause persistent symptoms, such as evere mosaic, mottling, malformation of leaves and pods (type strain); or severe stunting and blistering of leaves (Ampollado strain); or symmetric mosaic patterns on leaves.

References:
1. 
Van Kammen, A. and de Jager, C.P. (1978). CMI/AAB Descr. Pl. Viruses No. 197, 6 pp.
2.  Gamez, R. (1982). CMI/AAB Descr. Pl. Viruses No. 246, 4 pp.


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