Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Broccoli Necrotic Yellows Virus (BNYV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V187
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all BNYV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.750 - 2.850 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues such as asparagus and other host plants. Background is relative low on all of the negative control wells.
Application: The test can be used to detect BNYV in infected host plants.
Products:
|
Catalog Number |
Description | Test Wells | Price $US |
Ship
Weight (Estimated) |
| V187-R1 | ELISA Reagents, Alkaline Phosphatase |
500 |
240 |
2 lb (1 kg) |
| V187-R2 | ELISA Reagents, Alkaline Phosphatase |
1000 |
415 |
3 lb (1.5 kg) |
| V187-R3 | ELISA Reagents, Alkaline Phosphatase |
5000 |
1660 |
12 lb (6 kg) |
| V187-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
95 |
1 lb (0.5 kg) |
| V187-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
355 |
2 lb (1 kg) |
| V187-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
565 |
3 lb (1.5 kg) |
| V187-C1 | Coating Antibody |
500 |
120 |
2 lb (1 kg) |
| V187C2 | Coating Antibody |
1000 |
215 |
2 lb (1 kg) |
| V187-C3 | Coating Antibody |
5000 |
750 |
2 lb (1 kg) |
| V187-D1 | Detecting conjugate, Alkaline Phosphatase |
500 |
155 |
2 lb (1 kg) |
| V187-D2 | Detecting conjugate, Alkaline Phosphatase |
1000 |
255 |
2 lb (1 kg) |
| V187-D3 | Detecting conjugate, Alkaline Phosphatase |
5000 |
960 |
2 lb (1 kg) |
| V187-P2 | ELISA Control, Positive |
18 |
15 |
1 lb (0.5 kg) |
| V187-N1 | Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 |
3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 |
6 lb (3.0 kg) |
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An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Broccoli Necrotic Yellows Virus
Acronym: BNYV
Synonyms:
Group/Genus: cytorhabdovirus
Vector: Transmitted by a vector; an insect; Brevicoryne brassicae
(Tomlinson, Webb and Faithfull, 1972); Aphididae.
Transmission: Virus transmitted by mechanical inoculation; not
transmitted by seed.
Main host plants: Brassica oleracea var. botrytis
(broccoli), Brassica oleracea var. gemmifera (brussel sprouts)
Virus Infection: symptomless.
Diagnostically susceptible host species and symptoms: Brassica oleracea
- vein-clearing and leaf-rolling, later symptomless. Datura stramonium -
chlorotic and necrotic local lesions, then systemic mosaic and necrosis of
veins. Nicotiana glutinosa -sometimes local lesions, then systemic
mosaic.
References:
1. Campbell, R.N. and Lin, M.T. (1972). CMI/AAB Descr. Pl. Viruses
No. 85, 4 pp.
2. Garret, R.G. and O'Loughlin, G.T. (1976). Virology 76:
653.