Broccoli Necrotic Yellows Virus (BNYV) - DAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V187
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all BNYV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.750 - 2.850 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as asparagus and other host plants. Background is relative low on all of  the negative control wells.

Application:  The test can be used to detect BNYV in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)
V187-R1 ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)
V187-R2 ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)
V187-R3 ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V187-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V187-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V187-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)
V187-C1 Coating Antibody

500

125

2 lb (1 kg)
V187C2 Coating Antibody

1000

235

2 lb (1 kg)
V187-C3 Coating Antibody

5000

805

2 lb (1 kg)
V187-D1 Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)
V187-D2 Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)
V187-D3 Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)
V187-P2 ELISA Control, Positive

18

18

1 lb (0.5 kg)
V187-N1 Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Broccoli Necrotic Yellows Virus
Acronym:  BNYV
Synonyms: 
Group/Genus:  cytorhabdovirus

Vector: Transmitted by a vector; an insect; Brevicoryne brassicae (Tomlinson, Webb and Faithfull, 1972); Aphididae.
Transmission: Virus transmitted by mechanical inoculation; not transmitted by seed.
Main host plants: Brassica oleracea var. botrytis (broccoli), Brassica oleracea var. gemmifera (brussel sprouts)

Virus Infection: symptomless.
Diagnostically susceptible host species and symptoms:
Brassica oleracea - vein-clearing and leaf-rolling, later symptomless. Datura stramonium - chlorotic and necrotic local lesions, then systemic mosaic and necrosis of veins. Nicotiana glutinosa -sometimes local lesions, then systemic mosaic.

Geographical distribution: Spreads in Australia and the UK.

 References:
1. 
Campbell, R.N. and Lin, M.T. (1972). CMI/AAB Descr. Pl. Viruses No. 85, 4 pp.
2.  Garret, R.G. and O'Loughlin, G.T. (1976). Virology 76: 653.


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