Bean Golden Mosaic Virus (BGMV) - DAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V147
Source Antibody:  A rabbit polyclonal antibody (DSMZ AS-0141) for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all BGMV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.700 - >1.900 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:270 -1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as bean or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect BGMV in infected bean and other infected host plant tissues.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V147-R1

ELISA Reagents, Alkaline Phosphatase

500

240

2 lb (1 kg)

V147-R2

ELISA Reagents, Alkaline Phosphatase

1000

415

3 lb (1.5 kg)

V147-R3

ELISA Reagents, Alkaline Phosphatase

5000

1660

12 lb (6 kg)
V147-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V147-K1 ELISA Kit, Alkaline Phosphatase

500

365

2 lb (1 kg)
V147-K2 ELISA Kit, Alkaline Phosphatase

1000

575

3 lb (1.5 kg)

V147-C1

Coating Antibody

500

120

2 lb (1 kg)

V147-C2

Coating Antibody

1000

215

2 lb (1 kg)

V147-C3

Coating Antibody

5000

750

2 lb (1 kg)

V147-D1

Detecting conjugate, Alkaline Phosphatase

500

155

2 lb (1 kg)

V147-D2

Detecting conjugate, Alkaline Phosphatase

1000

255

2 lb (1 kg)

V147-D3

Detecting conjugate, Alkaline Phosphatase

5000

960

2 lb (1 kg)

V147-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V147-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase    1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase
5000
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Bean Golden Mosaic Virus
Acronym:  BGMV
Synonyms:  virus del mosaico dorado
Group/Genus:  Bigeminivirus

Vector:  Transmitted by a vector; an insect; Bemisia tabaci (Sida race); Aleyrodidae. Transmitted in a persistent manner. Virus retained when the vector moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector.
Transmission: Some isolates transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.
Main natural host plants: Macroptilium lathyroides, Phaseolus vulgaris, P. lunatus, Malvastrum coromandelianum.

Virus Infection:  Symptoms caused by viral infection include vein yellow net, interveinal chlorosis. Symptoms persist.
Diagnostically susceptible host species and symptoms: Phaseolus vulgaris cv. Top Crop, Phaseolus lunatus - systemic vein chlorosis, golden mosaic.

Geographical distribution: Spreads in Brazil, the Dominican Republic, Guatemala, Jamaica, Puerto Rico, and Venezuela.

References:
1. 
Goodman, R.M. and Bird, J. (1978). CMI/AAB Descr. Pl. Viruses No. 192, 4 pp.
2.  Howarth, A.J. and Vandemark, G.J. (1989). J. gen. Virol. 70: 2717.
3.  Kim, K.S., Shock, T.L. and Goodman, R.M. (1978). Virology 89: 22


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