Broad Bean Stain Virus (BBSV) - DAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V143
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all BBSV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405_nm  is in arrange of 0.800 - >2.200 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as bean, pea, cowpea or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect BBSV in infected host plants.

Products: 

It is easy and convenient To Place An Order
To know more about the products:  Product Items
To learn how to perform the test:  Instructions for DAS ELISA  In PDF Format  In PUB Format
To be familiar with other components: Buffer Sets - PDF PUB; Controls - PDF PUB

Information About the Virus

Name:  Broad Bean Stain Virus
Acronym:  BBSV
Synonyms:  virus de la mosaique de la fève, MF virus, broad bean F1 virus (Gibbs et al., 1968), broad bean Evesham stain virus
Group/Genus:  Comovirus

Vector:  Transmitted by a vector; an insect; Apion vorax, Sitona spp.; Coleoptera.
Transmission: Virus transmitted by mechanical inoculation; transmitted by grafting; transmitted by seed (up to 10% in some Vicia faba cultivars).
Main natural host plants: Vicia faba

Virus Infection:  Symptoms include systemic mottling, necrosis and malformation. Symptoms vary cyclically over a few weeks, or vary seasonally.
Diagnostically susceptible host species and symptoms: Phaseolus vulgaris cv. Tendergreen - systemic mottle; Pisum sativum, Vicia faba - systemic mottle; necrosis.

Geographical distribution: Spreads in the African region and the Eurasian region. Found, but with no evidence of spread, in Australia from imported seed.

References:
1.  Gibbs, A.J. and Smith, H.G. (1970). CMI/AAB Descr. Pl. Viruses No. 29, 3 pp.
2.  Russo, M., Castellano, M.A. and Martelli, G.P. (1982). J. Submicrosc. Cytol. 14: 149.

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