Broad Bean Stain Virus (BBSV) - DAS ELISA                           Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V143
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all BBSV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.800 - >2.200 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as bean, pea, cowpea or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect BBSV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V143-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V143-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V143-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V143-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V143-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V143-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V143-C1

Coating Antibody

500

125

2 lb (1 kg)

V143-C2

Coating Antibody

1000

235

2 lb (1 kg)

V143-C3

Coating Antibody

5000

805

2 lb (1 kg)

V143-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V143-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V143-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V143-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V143-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Broad Bean Stain Virus
Acronym:  BBSV
Synonyms:  virus de la mosaique de la fève, MF virus, broad bean F1 virus (Gibbs et al., 1968), broad bean Evesham stain virus
Group/Genus:  Comovirus

Vector:  Transmitted by a vector; an insect; Apion vorax, Sitona spp.; Coleoptera.
Transmission: Virus transmitted by mechanical inoculation; transmitted by grafting; transmitted by seed (up to 10% in some Vicia faba cultivars).
Main natural host plants: Vicia faba

Virus Infection:  Symptoms include systemic mottling, necrosis and malformation. Symptoms vary cyclically over a few weeks, or vary seasonally.
Diagnostically susceptible host species and symptoms: Phaseolus vulgaris cv. Tendergreen - systemic mottle; Pisum sativum, Vicia faba - systemic mottle; necrosis.

Geographical distribution: Spreads in the African region and the Eurasian region. Found, but with no evidence of spread, in Australia from imported seed.

References:
1. 
Gibbs, A.J. and Smith, H.G. (1970). CMI/AAB Descr. Pl. Viruses No. 29, 3 pp.
2.  Russo, M., Castellano, M.A. and Martelli, G.P. (1982). J. Submicrosc. Cytol. 14: 149.


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