Broad Bean Mottle Virus (BBMV) - DAS ELISA                          Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V175
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all BBMV isolates tested. Reaction of the ELISA is strong.  Optical Density at 405nm  is in arrange of 1.000 - >3.000 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is  high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as bean and soybean. Background is very low on all of  the negative control wells.

Application:  The test can be used to detect BBMV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V175-R1

ELISA Reagents, Alkaline Phosphatase

500

250

2 lb (1 kg)

V175-R2

ELISA Reagents, Alkaline Phosphatase

1000

425

3 lb (1.5 kg)

V175-R3

ELISA Reagents, Alkaline Phosphatase

5000

1690

12 lb (6 kg)
V175-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V175-K1 ELISA Kit, Alkaline Phosphatase

500

395

2 lb (1 kg)
V175-K2 ELISA Kit, Alkaline Phosphatase

1000

620

3 lb (1.5 kg)

V175-C1

Coating Antibody

500

125

2 lb (1 kg)

V175-C2

Coating Antibody

1000

235

2 lb (1 kg)

V175-C3

Coating Antibody

5000

805

2 lb (1 kg)

V175-D1

Detecting conjugate, Alkaline Phosphatase

500

170

2 lb (1 kg)

V175-D2

Detecting conjugate, Alkaline Phosphatase

1000

285

2 lb (1 kg)

V175-D3

Detecting conjugate, Alkaline Phosphatase

5000

980

2 lb (1 kg)

V175-P1

Positive control

18

18

1 lb (0.5 kg)

V175-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Broad Bean Mottle Virus
Acronym:  BBMV
Synonyms:  
Group/Genus:  Bromoviruses

Vector:  Transmitted by a vector; an insect; Acalymma trivittata, Colaspis flavida, Diabrotica undecimpunctata, Sitona lineata; Coleoptera.
Transmission: Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by seed.
Main host plants:  Vicia faba

Virus Infection:  Cause symptoms of leaf mottling and malformation. Symptoms persist.
Diagnostically susceptible host species and symptoms:
Chenopodium amaranticolor - chlorotic local lesions; not systemic; Cyamopsis tetragonoloba - necrotic local lesions or streaks; Phaseolus vulgaris cv. Canadian Wonder - chlorotic local lesions; systemic mottle and vein yellowing or netting; Vicia faba - systemic blotchy mottle

Geographical distribution: Spreads in Portugal, Sudan, and the UK.

 References:
1. 
Lane, L.C. (1979). CMI/AAB Descr. Pl. Viruses No. 215, 4 pp.
2.  Dzianott, A.M. and Bujarski, J.J. (1991). Virology 185: 553.


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