Apple Mosaic Virus (ApMV) - DAS ELISA                                Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V003
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This ELISA detects all strains known as apple mosaic or rose mosaic tested.  Reaction of the ELISA is moderately strong.  Optical Density at 405_nm is in arrange of 0.600 - 1.400 depending on the virus titer in the samples tested. For obtaining good test result, special attention should be given to the grinding and extraction of the sample.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant tissues diluted at 1:810.

Specificity:  This test system has no cross reaction with healthy plant tissues such as 
apple and rose. Background is low on all of the negative control wells.

Products: 

It is easy and convenient To Place An Order
To know more about the products:  Product Items
To learn how to perform the test:  Instructions for DAS ELISA Reagents
To learn how to perform the Pathogen ID test: Instructions for DAS ELISA Reagents
To be familiar with other components: Buffer Sets - PDF PUB; Controls - PDF PUB

Information About the Virus

Name: Apple Mosaic Virus
Acronym:  ApMV
Synonyms: Apple infectious variegation virus, European plum line pattern virus, Plum line pattern virus, Rose infectious chlorosis virus, Rose mosaic virus
Group/Genus:  Ilarviruses

Vector:  Unknown
Transmission:  Grafting, mechanical inoculation
Main host plants: Apple, rose, hops, birch and plum

Virus Infection:  Apple mosaic virus (ApMV) is a small, isometric, RNA virus which is mainly spread by grafting propagation of host plants.  The virus is easily transmitted by mechanical inoculation among herbaceous hosts although it is difficult to transmit from woody to herbaceous plants.

ApMV causes a variety of leaf symptoms and reductions in fruit yield of varying severity in apple. Typical symptoms of ApMV infection in rose is leaf mosaic.  Other symptoms include line patterns, ringspots, and mottle in infected rose leaves.  A good indexing program is the most effective method to control the viral infection.

References:
1.  CMI/AAB Descriptions of Plant Viruses, No. 83.
2. Howell, W. E., C. L. Parish, and G. I. Mink. 1990. In A. L. Jones and H. S. Aldwinckle, ed. Compendium of Apple and Pear Diseases. APS Press, St. Paul. (ISBN 0-89054-109-4.

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