Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Asparagus Virus - 2 (AV-2) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V205
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all AV-2 isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.860 - 2.530 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues such as asparagus and other host plants. Background is relative low on all of the negative control wells.
Application: The test can be used to detect AV-2 in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells |
Price |
Ship
Weight (Estimated) |
| V205-R1 | ELISA Reagents, Alkaline Phosphatase |
500 |
230 |
2 lb (1 kg) |
| V205-R2 | ELISA Reagents, Alkaline Phosphatase |
1000 |
395 |
3 lb (1.5 kg) |
| V205-R3 | ELISA Reagents, Alkaline Phosphatase |
5000 |
1590 |
12 lb (6 kg) |
| V205-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
85 |
1 lb (0.5 kg) |
| V205-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
335 |
2 lb (1 kg) |
| V205-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
525 |
3 lb (1.5 kg) |
| V205-C1 | Coating Antibody |
500 |
115 |
2 lb (1 kg) |
| V205-C2 | Coating Antibody |
1000 |
205 |
2 lb (1 kg) |
| V205-C3 | Coating Antibody |
5000 |
725 |
2 lb (1 kg) |
| V184-D1 | Detecting conjugate, Alkaline Phosphatase |
500 |
140 |
2 lb (1 kg) |
| V205-D2 | Detecting conjugate, Alkaline Phosphatase |
1000 |
225 |
2 lb (1 kg) |
| V205-D3 | Detecting conjugate, Alkaline Phosphatase |
5000 |
900 |
2 lb (1 kg) |
| V205-P2 | ELISA Control, Positive |
18 |
12 |
1 lb (0.5 kg) |
| V205-N1 | Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 80 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 105 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 335 | 6 lb (3.0 kg) |
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To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer Sets -
PDF PUB; Controls -
PDF PUB
Information About the Virus
Name: Asparagus Virus - 2
Acronym: AV-2
Synonyms: asparagus virus C, asparagus latent virus.
Group/Genus: Ilarvirus
Vector: Not transmitted by Myzus persicae.
Transmission: Not transmitted by Myzus persicae. Virus transmitted
by mechanical inoculation; transmitted by grafting; transmitted by seed;
transmitted by pollen to the seed and transmitted by pollen to the pollinated
plant.
Main natural host plants: Asparagus officinalis
Virus Infection: Cause stunting, decline of plants. Symptoms persist
Diagnostically susceptible host species and symptoms: Chenopodium
amaranticolor, C. quinoa - local necrotic spots, systemic necrosis;
Gomphrena globosa - white spots with red borders; Nicotiana tabacum
cv. Samsun - local necrotic rings and systemic vein yellowing and necrotic
lines; Nicotiana tabacum cv. White Burley - local necrotic rings.
References:
1. Bertaccini, A., Giunchedi, L. and Poggi Pollini, C. (1990). Acta
Hort. 271: 279.
2. Uyeda, I. and Mink, G.I. (1984). CMI/AAB Descr. Pl. Viruses
No.288, 4 pp.