Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Asparagus Virus - 1 (AV-1) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V184
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all AV-1 isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.750 - 2.850 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues such as asparagus and other host plants. Background is relative low on all of the negative control wells.
Application: The test can be used to detect AV-1 in infected host plants.
Products:
|
Catalog Number |
Description | Test Wells | Price $US |
Ship
Weight (Estimated) |
| V184-R1 | ELISA Reagents, Alkaline Phosphatase |
500 |
240 |
2 lb (1 kg) |
| V184-R2 | ELISA Reagents, Alkaline Phosphatase |
1000 |
415 |
3 lb (1.5 kg) |
| V184-R3 | ELISA Reagents, Alkaline Phosphatase |
5000 |
1660 |
12 lb (6 kg) |
| V184-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
95 |
1 lb (0.5 kg) |
| V184-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
355 |
2 lb (1 kg) |
| V184-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
565 |
3 lb (1.5 kg) |
| V184-C1 | Coating Antibody |
500 |
120 |
2 lb (1 kg) |
| V184C2 | Coating Antibody |
1000 |
215 |
2 lb (1 kg) |
| V184-C3 | Coating Antibody |
5000 |
750 |
2 lb (1 kg) |
| V184-D1 | Detecting conjugate, Alkaline Phosphatase |
500 |
155 |
2 lb (1 kg) |
| V184-D2 | Detecting conjugate, Alkaline Phosphatase |
1000 |
255 |
2 lb (1 kg) |
| V184-D3 | Detecting conjugate, Alkaline Phosphatase |
5000 |
960 |
2 lb (1 kg) |
| V184-P2 | ELISA Control, Positive |
18 |
15 |
1 lb (0.5 kg) |
| V184-N1 | Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 90 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 120 |
3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 350 |
6 lb (3.0 kg) |
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To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer Sets -
Buffer
Sets; Controls
Information About the Virus
Name: Asparagus Virus - 1
Acronym: AV-1
Synonyms: asparagus virus B.
Group/Genus: Potyvirus
Vector: Transmitted by a vector; an insect; Aphis craccivora, Myzus
persicae; Aphididae. Principal natural vector(s): Myzus persicae. Not
transmitted by Aphis gossypii, Macrosiphum euphorbiae. Transmitted in a
non-persistent manner.
Transmission: Virus transmitted by mechanical inoculation; not
transmitted by contact between plants; not transmitted by seed; not transmitted
by pollen.
Main host plants: Symptoms none. Asparagus officinalis
Virus Infection: symptomless.
Diagnostically susceptible host species and symptoms: Chenopodium album,
C. amaranticolor, C. quinoa, Tetragonia tetragonioides - chlorotic or
necrotic local lesions.
References:
1. Bertaccini, A., Giuncheoli, L. and Poggi Pollini, C. (1990).
Acta Hort. 271: 279.
2. Evans, T.A. and Stephens, C.T. (1989). Phytopathology
79: 253.