Apple Stem Grooving Virus (ASGV) - DAS ELISA                 Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V203
Source Antibody:  A rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all ASGV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405_nm  is in arrange of 0.400 - >1.700 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:30 -1:90.

Specificity:  There is no cross reaction with healthy plant  tissues such as apple or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect ASGV in infected herbaceous host plant tissues. Detection of ASGV in infected woody plant tissues are not consistent, and are depending on the viral titers in the sample tissues.

Products: 

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To know more about the products:  Product Items
To learn how to perform the test: Instructions for DAS ELISA
To be familiar with other components: Buffer Sets - PDF PUB; Controls - PDF PUB

Information About the Virus

Name:  Apple Stem Grooving Virus
Acronym:  ASGV
Synonyms:  Virginia Crab stem grooving virus, chenopodium dark green epinasty virus, brown line disease virus.
Group/Genus:  Capillovirus

Vector:  Transmitted by means not involving a vector.
Transmission: Virus transmitted by mechanical inoculation (from apple, especially in spring, by inoculating extracts from buds, young leaves or petals ground in 0.05 M phosphate buffer, pH 7-8. Additives such as 2% (w/v) nicotine base or 2% (w/v) PVP or hide powder help but are not essential); transmitted by grafting; transmitted by seed (in Chenopodium quinoa).
Main natural host plants: Malus sylvestris 

Virus Infection Symptoms: Virginia Crab Apple- stem grooves, abnormal graft union.  Symptoms in Virginia Crab apple persist.
Diagnostically susceptible host species and symptoms: Malus sylvestris cv. Virginia Crab - grooves in the wood. Strain `E-36' causes graft union to be brown, fragile, swollen and sunken areas on the stem; Chenopodium quinoa - etched necrotic local lesions, systemic chlorotic rings and mottle, tip malformed; some isolates induce epinasty; Nicotiana glutinosa - systemic yellow mosaic, line patterns; Phaseolus vulgaris - purple brown spots, chlorotic local lesions, systemic necrosis.

Geographical distribution: Probably distributed worldwide. Spreads in Australia, China, India, Italy, Japan, the Netherlands, New Zealand, Portugal, and the UK (probably occurs wherever apple trees are cultivated).

References:
1.  de Sequeira, O.A. and Lister, R.M. (1969b). Phytopathology 59: 1740.
2.  Lister, R.M. (1970). CMI/AAB Descr. Pl. Viruses No. 31, 4 pp.
3.  Yoshikawa, N. Sasaki, E., Kato, M. and Takahashi, T. (1992). Virology 191: 98.

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