Apple Stem Grooving Virus (ASGV) - DAS ELISA Home Products
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Catalog #: V203
Source Antibody: A rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA

Reactivity: The test reacted with all ASGV isolates tested.
Reaction of the ELISA is moderate strong. Optical Density at 405nm
is in arrange of 0.400 - >1.700 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high.
The virus can be consistently detected in infected plant tissues diluted
at 1:30 -1:90.
Specificity: There is no cross reaction with healthy plant
tissues such as apple or other host plants. Background is
low on all of the
negative control wells.
Application: The test can be used to detect ASGV in infected
herbaceous host plant tissues. Detection of ASGV in infected woody plant
tissues are not consistent, and are depending on the viral titers in the sample
tissues.
Products:
|
Catalog Number |
Description |
Test
Wells
|
Price
$US
|
Ship
Weight
(Estimated) |
|
V203-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
230 |
2 lb (1 kg) |
|
V203-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
395 |
3 lb (1.5 kg) |
|
V203-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1590 |
12 lb (6 kg) |
|
V203-R4 |
ELISA Reagents, Alkaline Phosphatase |
100 |
85 |
1 lb (0.5 kg) |
|
V203-K1 |
ELISA Kit, Alkaline Phosphatase |
500 |
335 |
2 lb (1 kg) |
|
V203-K2 |
ELISA Kit, Alkaline Phosphatase |
1000 |
525 |
3 lb (1.5 kg) |
|
V203-C1 |
Coating Antibody |
500 |
115 |
2 lb (1 kg) |
|
V203-C2 |
Coating Antibody |
1000 |
205 |
2 lb (1 kg) |
|
V203-C3 |
Coating Antibody |
5000 |
725 |
2 lb (1 kg) |
|
V203-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
140 |
2 lb (1 kg) |
|
V203-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
225 |
2 lb (1 kg) |
|
V203-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
900 |
2 lb (1 kg) |
|
V203-P2 |
ELISA control, positive |
18 |
15 |
1 lb (0.5 kg) |
|
V203-N1 |
Negative control |
18 |
15 |
1 lb (0.5 kg) |
| M004-1 |
Buffers for DAS/TAS, alkaline
phosphatase |
500 |
90 |
2
lb (1.0 kg) |
| M004-2 |
Buffers for DAS/TAS, alkaline
phosphatase |
1000 |
120 |
3
lb (1.5 kg) |
| M004-3 |
Buffers for DAS/TAS, alkaline
phosphatase |
5000 |
350 |
6
lb (3.0 kg) |
It is easy and convenient To Place
An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions for DAS
ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Apple Stem Grooving Virus
Acronym: ASGV
Synonyms: Virginia Crab stem grooving virus, chenopodium dark green
epinasty virus, brown line disease virus.
Group/Genus: Capillovirus
Vector: Transmitted by means not involving a vector.
Transmission: Virus transmitted by mechanical inoculation (from apple,
especially in spring, by inoculating extracts from buds, young leaves or petals
ground in 0.05 M phosphate buffer, pH 7-8. Additives such as 2% (w/v) nicotine
base or 2% (w/v) PVP or hide powder help but are not essential); transmitted by
grafting; transmitted by seed (in Chenopodium quinoa).
Main natural host plants: Malus sylvestris
Virus Infection Symptoms: Virginia Crab Apple- stem
grooves, abnormal graft union. Symptoms in Virginia Crab apple persist.
Diagnostically susceptible host species and symptoms: Malus sylvestris
cv. Virginia Crab - grooves in the wood. Strain `E-36' causes graft union to be
brown, fragile, swollen and sunken areas on the stem; Chenopodium quinoa
- etched necrotic local lesions, systemic chlorotic rings and mottle, tip
malformed; some isolates induce epinasty; Nicotiana glutinosa - systemic
yellow mosaic, line patterns; Phaseolus vulgaris - purple brown spots,
chlorotic local lesions, systemic necrosis.
Geographical distribution: Probably
distributed worldwide. Spreads in Australia, China, India, Italy, Japan, the
Netherlands, New Zealand, Portugal, and the UK (probably occurs wherever apple
trees are cultivated).
References:
1. de Sequeira, O.A. and Lister, R.M. (1969b).
Phytopathology 59: 1740.
2. Lister, R.M. (1970). CMI/AAB Descr. Pl. Viruses No. 31, 4
pp.
3. Yoshikawa, N. Sasaki, E., Kato, M. and Takahashi, T. (1992).
Virology 191: 98.
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