Apple Chlorotic Leaf Spot Virus (ACLSV) - DAS ELISA                 Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V148
Source Antibody:  A rabbit polyclonal antibody (DSMZ AS-0192) for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all ACLSV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.300 - >1.800 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:90 -1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as apple or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect ACLSV in infected herbaceous host plant tissues. Detection of ACLSV in infected woody plant tissues are not consistent, and are depending on the viral titers in the sample tissues.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V148-R1

ELISA Reagents, Alkaline Phosphatase

500

240

2 lb (1 kg)

V148-R2

ELISA Reagents, Alkaline Phosphatase

1000

415

3 lb (1.5 kg)

V148-R3

ELISA Reagents, Alkaline Phosphatase

5000

1660

12 lb (6 kg)
V148-R4 ELISA Reagents, Alkaline Phosphatase

100

95

1 lb (0.5 kg)
V148-K1 ELISA Kit, Alkaline Phosphatase

500

365

2 lb (1 kg)
V148-K2 ELISA Kit, Alkaline Phosphatase

1000

575

3 lb (1.5 kg)

V148-C1

Coating Antibody

500

120

2 lb (1 kg)

V148-C2

Coating Antibody

1000

215

2 lb (1 kg)

V148-C3

Coating Antibody

5000

750

2 lb (1 kg)

V148-D1

Detecting conjugate, Alkaline Phosphatase

500

155

2 lb (1 kg)

V148-D2

Detecting conjugate, Alkaline Phosphatase

1000

255

2 lb (1 kg)

V148-D3

Detecting conjugate, Alkaline Phosphatase

5000

960

2 lb (1 kg)

V148-P2

ELISA control, positive

18

18

1 lb (0.5 kg)

V148-N1

Negative control

18

18

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
100
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
130
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
400
6 lb (3.0 kg)

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To know more about the products:
  Product Items
To learn how to perform the test:
Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Apple Chlorotic Leaf Spot Virus
Acronym:  ACLSV
Synonyms:  pear ring pattern mosaic virus (Cropley, 1969), apple latent virus type 1, plum pseudopox virus, quince stunt virus.
Group/Genus:  Trichovirus

Vector:  Transmitted by eudorylamoid nematodes (Fritzsche and Kegler, 1968); an unconfirmed report.
Transmission: transmitted by mechanical inoculation; transmitted by grafting (possibly by root-grafts; Gilmer et al., 1971); not transmitted by seed.
Main natural host plants: Malus platycarpa, Malus sylvestris cv. R12740-7A, Malus sylvestris cv. Spy, Malus sylvestris cv. Virginia Crab - stem pitting, Cydonia oblonga (Quince) C7/1, Pyronia veitchii - chlorosis and stunting, Pyrus communis - ring patterns, mosaic, Crataegus spp. - chlorotic ring spots, Prunus armeniaca - ring patterns, mosaic, Prunus spinosa - chlorotic ringspot patterns, Prunus persica, Prunus domestica and Prunus armeniaca.

Virus Infection:  Symptoms caused by viral infection include chlorotic leaf spots and stem pitting, line patterns and chlorotic rings, chlorotic spots and stem pitting, chlorosis and stunting, dark green mottle, bark split and pseudopox.
Diagnostically susceptible host species and symptoms: Malus sylvestris cv. R12740-7A - asymmetric blotches and malformation of young leaves; M. platycarpa (Long Ashton clone) - irregular chlorotic rings and line patterns, small leaves shed prematurely, malformation; Chenopodium quinoa - large etched local lesions, becoming necrotic, systemic chlorotic spotting, mottling, ring and line patterns, chlorosis and necrosis; C. amaranticolor - chlorotic local lesions, surrounded by necrotic dots; Phaseolus vulgaris cvs Pinto, Bountiful, Kinghorn - purple-brown necrotic spots or rings (1-3 mm), not systemic..

Geographical distribution: Probably distributed worldwide (and probably wherever apples are cultivated). Spreads in the Eastern Asian region, the Eurasian region, the North American region, and the Pacific region; Australia, China, and New Zealand.

References:
1. 
German, S., Candresse, T., Le Gall, O., Lanneau, M. and Dunez, J. (1992). J. gen. Virol. 73: 767.
2.  Lister, R.M. (1970). CMI/AAB Descr. Pl. Viruses No. 30, 4 pp.
3.  Lister, R.M. and Bar-Joseph, M. (1981). Closteroviruses. In: Handbook of Plant Virus Infections and Comparative Diagnosis, p. 809; ed. E. Kurstak. Elsevier/North-Holland Biomedical Press.


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